TY - JOUR
T1 - Monitoring T-Cell Responses in Translational Studies: Optimization of Dye-Based Proliferation Assay for Evaluation of Antigen-Specific Responses
T2 - Optimization of Dye-Based Proliferation Assay for Evaluation of Antigen-Specific Responses
AU - ten Brinke, Anja
AU - Marek-Trzonkowska, Natalia
AU - Mansilla, Maria J.
AU - Turksma, Annelies W.
AU - Piekarska, Karolina
AU - Iwaszkiewicz-Grześ, Dorota
AU - Passerini, Laura
AU - Locafaro, Grazia
AU - Puñet-Ortiz, Joan
AU - van Ham, S. Marieke
AU - Hernandez-Fuentes, Maria P.
AU - Martínez-Cáceres, Eva M.
AU - Gregori, Silvia
PY - 2017
Y1 - 2017
N2 - Adoptive therapy with regulatory T cells or tolerance-inducing antigen (Ag)-presenting cells is innovative and promising therapeutic approach to control undesired and harmful activation of the immune system, as observed in autoimmune diseases, solid organ and bone marrow transplantation. One of the critical issues to elucidate the mechanisms responsible for success or failure of these therapies and define the specificity of the therapy is the evaluation of the Ag-specific T-cell responses. Several efforts have been made to develop suitable and reproducible assays. Here, we focus on dye-based proliferation assays. We highlight with practical examples the fundamental issues to take into consideration for implementation of an effective and sensitive dye-based proliferation assay to monitor Ag-specific responses in patients. The most critical points were used to design a road map to set up and analyze the optimal assay to assess Ag-specific T-cell responses in patients undergoing different treatments. This is the first step to optimize monitoring of tolerance induction, allowing comparison of outcomes of different clinical studies. The road map can also be applied to other therapeutic interventions, not limited to tolerance induction therapies, in which Ag-specific T-cell responses are relevant such as vaccination approaches and cancer immunotherapy
AB - Adoptive therapy with regulatory T cells or tolerance-inducing antigen (Ag)-presenting cells is innovative and promising therapeutic approach to control undesired and harmful activation of the immune system, as observed in autoimmune diseases, solid organ and bone marrow transplantation. One of the critical issues to elucidate the mechanisms responsible for success or failure of these therapies and define the specificity of the therapy is the evaluation of the Ag-specific T-cell responses. Several efforts have been made to develop suitable and reproducible assays. Here, we focus on dye-based proliferation assays. We highlight with practical examples the fundamental issues to take into consideration for implementation of an effective and sensitive dye-based proliferation assay to monitor Ag-specific responses in patients. The most critical points were used to design a road map to set up and analyze the optimal assay to assess Ag-specific T-cell responses in patients undergoing different treatments. This is the first step to optimize monitoring of tolerance induction, allowing comparison of outcomes of different clinical studies. The road map can also be applied to other therapeutic interventions, not limited to tolerance induction therapies, in which Ag-specific T-cell responses are relevant such as vaccination approaches and cancer immunotherapy
U2 - https://doi.org/10.3389/fimmu.2017.01870
DO - https://doi.org/10.3389/fimmu.2017.01870
M3 - Article
C2 - 29312346
SN - 1664-3224
VL - 8
SP - 1870
JO - Frontiers in immunology
JF - Frontiers in immunology
M1 - 1870
ER -