TY - JOUR
T1 - More severe cellular phenotype in human idiopathic dilated cardiomyopathy compared to ischemic heart disease
AU - Hamdani, N.
AU - Borbely, A.
AU - Veenstra, S.P.G.R.
AU - Kooij, V.
AU - Vrydag, W.
AU - Zaremba, R.
AU - dos Remedios, C.
AU - Niessen, H.W.M.
AU - Michel, M.C.
AU - Paulus, W.J.
AU - Stienen, G.J.M.
AU - van der Velden, J.
PY - 2010
Y1 - 2010
N2 - Activation of the beta-adrenergic receptor (beta AR) pathway is the main mechanism of the heart to increase cardiac output via protein kinase A (PKA)-mediated phosphorylation of cellular target proteins, and perturbations therein may contribute to cardiac dysfunction in heart failure. In the present study a comprehensive analysis was made of mediators of the beta AR pathway, myofilament properties and cardiac structure in patients with idiopathic (IDCM; n = 13) and ischemic (ISHD; n = 10) cardiomyopathy in comparison to non-failing hearts (donor; n = 10) for the following parameters: beta AR density, G-coupled receptor kinases 2 and 5, stimulatory and inhibitory G-proteins, phosphorylation of myofilament targets of PKA, protein phosphatase 1, phospholamban, SERCA2a and single myocyte contractility. All parameters exhibited the expected alterations of heart failure, but for most of them the extent of alteration was greater in IDCM than in ISHD. Histological analysis also revealed higher collagen in IDCM compared to ISHD. Alterations in the beta AR pathway are more pronounced in IDCM than in ISHD and may reflect sequential changes in cellular protein composition and function. Our data indicate that cellular dysfunction is more severe in IDCM than in ISHD
AB - Activation of the beta-adrenergic receptor (beta AR) pathway is the main mechanism of the heart to increase cardiac output via protein kinase A (PKA)-mediated phosphorylation of cellular target proteins, and perturbations therein may contribute to cardiac dysfunction in heart failure. In the present study a comprehensive analysis was made of mediators of the beta AR pathway, myofilament properties and cardiac structure in patients with idiopathic (IDCM; n = 13) and ischemic (ISHD; n = 10) cardiomyopathy in comparison to non-failing hearts (donor; n = 10) for the following parameters: beta AR density, G-coupled receptor kinases 2 and 5, stimulatory and inhibitory G-proteins, phosphorylation of myofilament targets of PKA, protein phosphatase 1, phospholamban, SERCA2a and single myocyte contractility. All parameters exhibited the expected alterations of heart failure, but for most of them the extent of alteration was greater in IDCM than in ISHD. Histological analysis also revealed higher collagen in IDCM compared to ISHD. Alterations in the beta AR pathway are more pronounced in IDCM than in ISHD and may reflect sequential changes in cellular protein composition and function. Our data indicate that cellular dysfunction is more severe in IDCM than in ISHD
U2 - https://doi.org/10.1007/s10974-010-9231-8
DO - https://doi.org/10.1007/s10974-010-9231-8
M3 - Article
C2 - 21132354
SN - 0142-4319
VL - 31
SP - 289
EP - 301
JO - Journal of muscle research and cell motility
JF - Journal of muscle research and cell motility
IS - 4
ER -