TY - JOUR
T1 - Multi-level characterization of balanced inhibitory-excitatory cortical neuron network derived from human pluripotent stem cells
AU - Nadadhur, Aishwarya G.
AU - Melero, Javier Emperador
AU - Meijer, Marieke
AU - Schut, Desiree
AU - Jacobs, Gerbren
AU - Wan Li, Ka
AU - Hjorth, J. J.Johannes
AU - Meredith, Rhiannon M.
AU - Toonen, Ruud F.
AU - Van Kesteren, Ronald E.
AU - Smit, August B.
AU - Verhage, Matthijs
AU - Heine, Vivi M.
PY - 2017/6/1
Y1 - 2017/6/1
N2 - Generation of neuronal cultures from induced pluripotent stem cells (hiPSCs) serve the studies of human brain disorders. However we lack neuronal networks with balanced excitatory- inhibitory activities, which are suitable for single cell analysis. We generated low-density networks of hPSC-derived GABAergic and glutamatergic cortical neurons. We used two different co-culture models with astrocytes. We show that these cultures have balanced excitatory-inhibitory synaptic identities using confocal microscopy, electrophysiological recordings, calcium imaging and mRNA analysis. These simple and robust protocols offer the opportunity for single-cell to multi-level analysis of patient hiPSC-derived cortical excitatory- inhibitory networks; thereby creating advanced tools to study disease mechanisms underlying neurodevelopmental disorders.
AB - Generation of neuronal cultures from induced pluripotent stem cells (hiPSCs) serve the studies of human brain disorders. However we lack neuronal networks with balanced excitatory- inhibitory activities, which are suitable for single cell analysis. We generated low-density networks of hPSC-derived GABAergic and glutamatergic cortical neurons. We used two different co-culture models with astrocytes. We show that these cultures have balanced excitatory-inhibitory synaptic identities using confocal microscopy, electrophysiological recordings, calcium imaging and mRNA analysis. These simple and robust protocols offer the opportunity for single-cell to multi-level analysis of patient hiPSC-derived cortical excitatory- inhibitory networks; thereby creating advanced tools to study disease mechanisms underlying neurodevelopmental disorders.
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U2 - https://doi.org/10.1371/journal.pone.0178533
DO - https://doi.org/10.1371/journal.pone.0178533
M3 - Article
C2 - 28586384
SN - 1932-6203
VL - 12
JO - PLoS ONE
JF - PLoS ONE
IS - 6
M1 - 0178533
ER -
