Mycobacterium marinum MMAR_2380, a predicted transmembrane acyltransferase, is essential for the presence of the mannose cap on lipoarabinomannan

N.N. Driessen, E.J.M. Stoop, R. Ummels, S.S. Gurcha, A. K. Mishra, G. Larrouy-Maumus, J. Nigou, M. Gilleron, G. Puzo, J.J. Maaskant, M. Sparrius, G.S. Besra, W. Bitter, C.M.J.E. Vandenbroucke-Grauls, B.J. Appelmelk

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Abstract

Lipoarabinomannan (LAM) is a major glycolipid in the mycobacterial cell envelope. LAM consists of a mannosylphosphatidylinositol (MPI) anchor, a mannan core and a branched arabinan domain. The termini of the arabinan branches can become substituted with one to three α(1→2)-linked mannosyl residues, the mannose cap, producing ManLAM. ManLAM has been associated with a range of different immunomodulatory properties of Mycobacterium tuberculosis during infection of the host. In some of these effects, the presence of the mannose cap on ManLAM appears to be crucial for its activity. So far, in the biosynthesis of the mannose cap on ManLAM, two enzymes have been reported to be involved: a mannosyltransferase that adds the first mannosyl residue of the mannose caps to the arabinan domain of LAM, and another mannosyltransferase that elongates the mannose cap up to three mannosyl residues. Here, we report that a third gene is involved, MMAR-2380, which is the Mycobacterium marinum orthologue of Rv1565c. MMAR-2380 encodes a predicted transmembrane acyltransferase. In M. marinum ΔMMAR-2380, the LAM arabinan domain is still intact, but the mutant LAM lacks the mannose cap. Additional effects of mutation of MMAR-2380 on LAM were observed: a higher degree of branching of both the arabinan domain and the mannan core, and a decreased incorporation of [1,2-
Original languageEnglish
Pages (from-to)3492-3502
JournalMicrobiology
Volume156
DOIs
Publication statusPublished - 2010

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