Natural human plasmacytoid dendritic cells induce antigen-specific T-cell responses in melanoma patients

Jurjen tel, Erik H. J. G. Aarntzen, Tetsuro Baba, Gerty Schreibelt, Barbara M. Schulte, Daniel Benitez-Ribas, Otto C. Boerman, Sandra Croockewit, Wim J. G. Oyen, Michelle van Rossum, Gregor Winkels, Pierre G. Coulie, Cornelis J. A. Punt, Carl G. Figdor, I. Jolanda M. de Vries

Research output: Contribution to journalArticleAcademicpeer-review

250 Citations (Scopus)

Abstract

Vaccination against cancer by using dendritic cells has for more than a decade been based on dendritic cells generated ex vivo from monocytes or CD34(+) progenitors. Here, we report on the first clinical study of therapeutic vaccination against cancer using naturally occurring plasmacytoid dendritic cells (pDC). Fifteen patients with metastatic melanoma received intranodal injections of pDCs activated and loaded with tumor antigen-associated peptides ex vivo. In vivo imaging showed that administered pDCs migrated and distributed over multiple lymph nodes. Several patients mounted antivaccine CD4(+) and CD8(+) T-cell responses. Despite the limited number of administered pDCs, an IFN signature was observed after each vaccination. These results indicate that vaccination with naturally occurring pDC is feasible with minimal toxicity and that in patients with metastatic melanoma, it induces favorable immune responses
Original languageEnglish
Pages (from-to)1063-1075
JournalCancer Research
Volume73
Issue number3
DOIs
Publication statusPublished - 2013

Cite this