Nef induces multiple genes involved in cholesterol synthesis and uptake in human immunodeficiency virus type 1-infected T cells

Angélique B. van 't Wout, J. Victor Swain, Michael Schindler, Ushnal Rao, Melissa S. Pathmajeyan, James I. Mullins, Frank Kirchhoff

Research output: Contribution to JournalArticleAcademicpeer-review

81 Citations (Scopus)

Abstract

Several recent reports indicate that cholesterol might play an important role in human immunodeficiency virus type 1 (HIV-1) replication. We investigated the effects of HIV-1 infection on cholesterol biosynthesis and uptake using microarrays. HIV-1 increased gene expression of cholesterol genes in both transformed T-cell lines and primary CD4(+) T cells. Consistent with our microarray data, (14)C-labeled mevalonate and acetate incorporation was increased in HIV-1-infected cells. Our data also demonstrate that changes in cholesterol biosynthesis and uptake are only observed in the presence of functional Nef, suggesting that increased cholesterol synthesis may contribute to Nef-mediated enhancement of virion infectivity and viral replication
Original languageEnglish
Pages (from-to)10053-10058
JournalJournal of virology
Volume79
Issue number15
DOIs
Publication statusPublished - 2005

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