Neuron-Glia Interactions Increase Neuronal Phenotypes in Tuberous Sclerosis Complex Patient iPSC-Derived Models

Aishwarya G. Nadadhur, Mouhamed Alsaqati, Lisa Gasparotto, Paulien Cornelissen-Steijger, Eline van Hugte, Stephanie Dooves, Adrian J. Harwood, Vivi M. Heine

Research output: Contribution to JournalArticleAcademicpeer-review

44 Citations (Scopus)

Abstract

Nadadhur et al. generated TSC disease models using patient iPSCs. While neuron mono-cultures showed an increase in network activity and dendritic branching, only when co-cultured with oligodendrocytes (OLs), hypertrophy and axonal abnormalities were observed. Neuron-OL interactions, modulated by mTOR regulators, support use of mixed cultures for TSC disease modeling and drug development.

Original languageEnglish
Pages (from-to)42-56
Number of pages15
JournalStem Cell Reports
Volume12
Issue number1
DOIs
Publication statusPublished - 8 Jan 2019

Keywords

  • autism
  • co-culture
  • glia
  • iPSC
  • in vitro model
  • myelin
  • neuron
  • neuronal activity
  • oligodendrocyte
  • tuberous sclerosis complex

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