Neuron-Glia Interactions Increase Neuronal Phenotypes in Tuberous Sclerosis Complex Patient iPSC-Derived Models

Aishwarya G. Nadadhur; Mouhamed Alsaqati; Lisa Gasparotto; Paulien Cornelissen-Steijger; Eline van Hugte; Stephanie Dooves; Adrian J. Harwood; Vivi M. Heine

doi:https://doi.org/10.1016/j.stemcr.2018.11.019

Neuron-Glia Interactions Increase Neuronal Phenotypes in Tuberous Sclerosis Complex Patient iPSC-Derived Models

Aishwarya G. Nadadhur, Mouhamed Alsaqati, Lisa Gasparotto, Paulien Cornelissen-Steijger, Eline van Hugte, Stephanie Dooves, Adrian J. Harwood, Vivi M. Heine

Research output: Contribution to journal › Article › Academic › peer-review

56 Citations (Scopus)

Abstract

Nadadhur et al. generated TSC disease models using patient iPSCs. While neuron mono-cultures showed an increase in network activity and dendritic branching, only when co-cultured with oligodendrocytes (OLs), hypertrophy and axonal abnormalities were observed. Neuron-OL interactions, modulated by mTOR regulators, support use of mixed cultures for TSC disease modeling and drug development.

Original language	English
Pages (from-to)	42-56
Number of pages	15
Journal	Stem cell reports
Volume	12
Issue number	1
DOIs	https://doi.org/10.1016/j.stemcr.2018.11.019
Publication status	Published - 8 Jan 2019

Keywords

autism
co-culture
glia
iPSC
in vitro model
myelin
neuron
neuronal activity
oligodendrocyte
tuberous sclerosis complex

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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https://doi.org/10.1016/j.stemcr.2018.11.019

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6335594

Cite this

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title = "Neuron-Glia Interactions Increase Neuronal Phenotypes in Tuberous Sclerosis Complex Patient iPSC-Derived Models",

abstract = "Nadadhur et al. generated TSC disease models using patient iPSCs. While neuron mono-cultures showed an increase in network activity and dendritic branching, only when co-cultured with oligodendrocytes (OLs), hypertrophy and axonal abnormalities were observed. Neuron-OL interactions, modulated by mTOR regulators, support use of mixed cultures for TSC disease modeling and drug development.",

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author = "{G. Nadadhur}, Aishwarya and Mouhamed Alsaqati and Lisa Gasparotto and Paulien Cornelissen-Steijger and {van Hugte}, Eline and Stephanie Dooves and Harwood, {Adrian J.} and Heine, {Vivi M.}",

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AU - G. Nadadhur, Aishwarya

AU - Alsaqati, Mouhamed

AU - Gasparotto, Lisa

AU - Cornelissen-Steijger, Paulien

AU - van Hugte, Eline

AU - Dooves, Stephanie

AU - Harwood, Adrian J.

AU - Heine, Vivi M.

PY - 2019/1/8

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N2 - Nadadhur et al. generated TSC disease models using patient iPSCs. While neuron mono-cultures showed an increase in network activity and dendritic branching, only when co-cultured with oligodendrocytes (OLs), hypertrophy and axonal abnormalities were observed. Neuron-OL interactions, modulated by mTOR regulators, support use of mixed cultures for TSC disease modeling and drug development.

AB - Nadadhur et al. generated TSC disease models using patient iPSCs. While neuron mono-cultures showed an increase in network activity and dendritic branching, only when co-cultured with oligodendrocytes (OLs), hypertrophy and axonal abnormalities were observed. Neuron-OL interactions, modulated by mTOR regulators, support use of mixed cultures for TSC disease modeling and drug development.

KW - autism

KW - co-culture

KW - glia

KW - iPSC

KW - in vitro model

KW - myelin

KW - neuron

KW - neuronal activity

KW - oligodendrocyte

KW - tuberous sclerosis complex

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Neuron-Glia Interactions Increase Neuronal Phenotypes in Tuberous Sclerosis Complex Patient iPSC-Derived Models

Abstract

Keywords

UN SDGs

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