Neuropilin-1 Is Expressed on Lymphoid Tissue Residing LTi-like Group 3 Innate Lymphoid Cells and Associated with Ectopic Lymphoid Aggregates

Medya Mara Shikhagaie; Åsa K. Björklund; Jenny Mjösberg; Jonas S. Erjefält; Anne S. Cornelissen; Xavier Romero Ros; Suzanne M. Bal; Jasper J. Koning; Reina E. Mebius; Michiko Mori; Melanie Bruchard; Bianca Blom; Hergen Spits

doi:https://doi.org/10.1016/j.celrep.2017.01.063

Neuropilin-1 Is Expressed on Lymphoid Tissue Residing LTi-like Group 3 Innate Lymphoid Cells and Associated with Ectopic Lymphoid Aggregates

Medya Mara Shikhagaie, Åsa K. Björklund, Jenny Mjösberg, Jonas S. Erjefält, Anne S. Cornelissen, Xavier Romero Ros, Suzanne M. Bal, Jasper J. Koning, Reina E. Mebius, Michiko Mori, Melanie Bruchard, Bianca Blom, Hergen Spits

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Abstract

Here, we characterize a subset of ILC3s that express Neuropilin1 (NRP1) and are present in lymphoid tissues, but not in the peripheral blood or skin. NRP1⁺ group 3 innate lymphoid cells (ILC3s) display in vitro lymphoid tissue inducer (LTi) activity. In agreement with this, NRP1⁺ ILC3s are mainly located in proximity to high endothelial venules (HEVs) and express cell surface molecules involved in lymphocyte migration in secondary lymphoid tissues via HEVs. NRP1 was also expressed on mouse fetal LTi cells, indicating that NRP1 is a conserved marker for LTi cells. Human NRP1⁺ ILC3s are primed cells because they express CD45RO and produce higher amounts of cytokines than NRP1⁻ cells, which express CD45RA. The NRP1 ligand vascular endothelial growth factor A (VEGF-A) served as a chemotactic factor for NRP1⁺ ILC3s. NRP1⁺ ILC3s are present in lung tissues from smokers and patients with chronic obstructive pulmonary disease, suggesting a role in angiogenesis and/or the initiation of ectopic pulmonary lymphoid aggregates.

Original language	English
Pages (from-to)	1761-1773
Number of pages	13
Journal	Cell reports
Volume	18
Issue number	7
DOIs	https://doi.org/10.1016/j.celrep.2017.01.063
Publication status	Published - 14 Feb 2017

Keywords

HEVs
ILC3
LA
LTi
NRP1
VEGF-A
chemotaxis
high endothelial venues
human innate lymphoid cells 3
lymphoid tissue inducer cells
neuropilin-1
smoke-induced pulmonary lymphoid aggregates
vascular endothelial growth factor A

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Shikhagaie, M. M., Björklund, Å. K., Mjösberg, J., Erjefält, J. S., Cornelissen, A. S., Ros, X. R., Bal, S. M., Koning, J. J., Mebius, R. E., Mori, M., Bruchard, M., Blom, B., & Spits, H. (2017). Neuropilin-1 Is Expressed on Lymphoid Tissue Residing LTi-like Group 3 Innate Lymphoid Cells and Associated with Ectopic Lymphoid Aggregates. Cell reports, 18(7), 1761-1773. https://doi.org/10.1016/j.celrep.2017.01.063

@article{d8f6034b47774375a5c0e9f2aab9fc33,

title = "Neuropilin-1 Is Expressed on Lymphoid Tissue Residing LTi-like Group 3 Innate Lymphoid Cells and Associated with Ectopic Lymphoid Aggregates",

abstract = "Here, we characterize a subset of ILC3s that express Neuropilin1 (NRP1) and are present in lymphoid tissues, but not in the peripheral blood or skin. NRP1+ group 3 innate lymphoid cells (ILC3s) display in vitro lymphoid tissue inducer (LTi) activity. In agreement with this, NRP1+ ILC3s are mainly located in proximity to high endothelial venules (HEVs) and express cell surface molecules involved in lymphocyte migration in secondary lymphoid tissues via HEVs. NRP1 was also expressed on mouse fetal LTi cells, indicating that NRP1 is a conserved marker for LTi cells. Human NRP1+ ILC3s are primed cells because they express CD45RO and produce higher amounts of cytokines than NRP1− cells, which express CD45RA. The NRP1 ligand vascular endothelial growth factor A (VEGF-A) served as a chemotactic factor for NRP1+ ILC3s. NRP1+ ILC3s are present in lung tissues from smokers and patients with chronic obstructive pulmonary disease, suggesting a role in angiogenesis and/or the initiation of ectopic pulmonary lymphoid aggregates.",

keywords = "HEVs, ILC3, LA, LTi, NRP1, VEGF-A, chemotaxis, high endothelial venues, human innate lymphoid cells 3, lymphoid tissue inducer cells, neuropilin-1, smoke-induced pulmonary lymphoid aggregates, vascular endothelial growth factor A",

author = "Shikhagaie, {Medya Mara} and Bj{\"o}rklund, {{\AA}sa K.} and Jenny Mj{\"o}sberg and Erjef{\"a}lt, {Jonas S.} and Cornelissen, {Anne S.} and Ros, {Xavier Romero} and Bal, {Suzanne M.} and Koning, {Jasper J.} and Mebius, {Reina E.} and Michiko Mori and Melanie Bruchard and Bianca Blom and Hergen Spits",

year = "2017",

month = feb,

day = "14",

doi = "https://doi.org/10.1016/j.celrep.2017.01.063",

language = "English",

volume = "18",

pages = "1761--1773",

journal = "Cell reports",

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Shikhagaie, MM, Björklund, ÅK, Mjösberg, J, Erjefält, JS, Cornelissen, AS, Ros, XR, Bal, SM, Koning, JJ , Mebius, RE, Mori, M, Bruchard, M, Blom, B & Spits, H 2017, 'Neuropilin-1 Is Expressed on Lymphoid Tissue Residing LTi-like Group 3 Innate Lymphoid Cells and Associated with Ectopic Lymphoid Aggregates', Cell reports, vol. 18, no. 7, pp. 1761-1773. https://doi.org/10.1016/j.celrep.2017.01.063

TY - JOUR

T1 - Neuropilin-1 Is Expressed on Lymphoid Tissue Residing LTi-like Group 3 Innate Lymphoid Cells and Associated with Ectopic Lymphoid Aggregates

AU - Shikhagaie, Medya Mara

AU - Björklund, Åsa K.

AU - Mjösberg, Jenny

AU - Erjefält, Jonas S.

AU - Cornelissen, Anne S.

AU - Ros, Xavier Romero

AU - Bal, Suzanne M.

AU - Koning, Jasper J.

AU - Mebius, Reina E.

AU - Mori, Michiko

AU - Bruchard, Melanie

AU - Blom, Bianca

AU - Spits, Hergen

PY - 2017/2/14

Y1 - 2017/2/14

N2 - Here, we characterize a subset of ILC3s that express Neuropilin1 (NRP1) and are present in lymphoid tissues, but not in the peripheral blood or skin. NRP1+ group 3 innate lymphoid cells (ILC3s) display in vitro lymphoid tissue inducer (LTi) activity. In agreement with this, NRP1+ ILC3s are mainly located in proximity to high endothelial venules (HEVs) and express cell surface molecules involved in lymphocyte migration in secondary lymphoid tissues via HEVs. NRP1 was also expressed on mouse fetal LTi cells, indicating that NRP1 is a conserved marker for LTi cells. Human NRP1+ ILC3s are primed cells because they express CD45RO and produce higher amounts of cytokines than NRP1− cells, which express CD45RA. The NRP1 ligand vascular endothelial growth factor A (VEGF-A) served as a chemotactic factor for NRP1+ ILC3s. NRP1+ ILC3s are present in lung tissues from smokers and patients with chronic obstructive pulmonary disease, suggesting a role in angiogenesis and/or the initiation of ectopic pulmonary lymphoid aggregates.

AB - Here, we characterize a subset of ILC3s that express Neuropilin1 (NRP1) and are present in lymphoid tissues, but not in the peripheral blood or skin. NRP1+ group 3 innate lymphoid cells (ILC3s) display in vitro lymphoid tissue inducer (LTi) activity. In agreement with this, NRP1+ ILC3s are mainly located in proximity to high endothelial venules (HEVs) and express cell surface molecules involved in lymphocyte migration in secondary lymphoid tissues via HEVs. NRP1 was also expressed on mouse fetal LTi cells, indicating that NRP1 is a conserved marker for LTi cells. Human NRP1+ ILC3s are primed cells because they express CD45RO and produce higher amounts of cytokines than NRP1− cells, which express CD45RA. The NRP1 ligand vascular endothelial growth factor A (VEGF-A) served as a chemotactic factor for NRP1+ ILC3s. NRP1+ ILC3s are present in lung tissues from smokers and patients with chronic obstructive pulmonary disease, suggesting a role in angiogenesis and/or the initiation of ectopic pulmonary lymphoid aggregates.

KW - HEVs

KW - ILC3

KW - LA

KW - LTi

KW - NRP1

KW - VEGF-A

KW - chemotaxis

KW - high endothelial venues

KW - human innate lymphoid cells 3

KW - lymphoid tissue inducer cells

KW - neuropilin-1

KW - smoke-induced pulmonary lymphoid aggregates

KW - vascular endothelial growth factor A

UR - http://www.scopus.com/inward/record.url?scp=85013188332&partnerID=8YFLogxK

U2 - https://doi.org/10.1016/j.celrep.2017.01.063

DO - https://doi.org/10.1016/j.celrep.2017.01.063

M3 - Article

C2 - 28199847

SN - 2211-1247

VL - 18

SP - 1761

EP - 1773

JO - Cell reports

JF - Cell reports

IS - 7

ER -

Neuropilin-1 Is Expressed on Lymphoid Tissue Residing LTi-like Group 3 Innate Lymphoid Cells and Associated with Ectopic Lymphoid Aggregates

Abstract

Keywords

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