TY - JOUR
T1 - NINscope, a versatile miniscope for multi-region circuit investigations
AU - de Groot, Andres
AU - van den Boom, Bastijn Jg
AU - van Genderen, Romano M.
AU - Coppens, Joris
AU - van Veldhuijzen, John
AU - Bos, Joop
AU - Hoedemaker, Hugo
AU - Negrello, Mario
AU - Willuhn, Ingo
AU - de Zeeuw, Chris I.
AU - Hoogland, Tycho M.
PY - 2020/1
Y1 - 2020/1
N2 - Miniaturized fluorescence microscopes (miniscopes) have been instrumental to monitor neural signals during unrestrained behavior and their open-source versions have made them affordable. Often, the footprint and weight of open-source miniscopes is sacrificed for added functionality. Here, we present NINscope: a light-weight miniscope with a small footprint that integrates a high-sensitivity image sensor, an inertial measurement unit and an LED driver for an external optogenetic probe. We use it to perform the first concurrent cellular resolution recordings from cerebellum and cerebral cortex in unrestrained mice, demonstrate its optogenetic stimulation capabilities to examine cerebello-cerebral or cortico-striatal connectivity, and replicate findings of action encoding in dorsal striatum. In combination with cross-platform acquisition and control software, our miniscope is a versatile addition to the expanding tool chest of open-source miniscopes that will increase access to multi-region circuit investigations during unrestrained behavior.
AB - Miniaturized fluorescence microscopes (miniscopes) have been instrumental to monitor neural signals during unrestrained behavior and their open-source versions have made them affordable. Often, the footprint and weight of open-source miniscopes is sacrificed for added functionality. Here, we present NINscope: a light-weight miniscope with a small footprint that integrates a high-sensitivity image sensor, an inertial measurement unit and an LED driver for an external optogenetic probe. We use it to perform the first concurrent cellular resolution recordings from cerebellum and cerebral cortex in unrestrained mice, demonstrate its optogenetic stimulation capabilities to examine cerebello-cerebral or cortico-striatal connectivity, and replicate findings of action encoding in dorsal striatum. In combination with cross-platform acquisition and control software, our miniscope is a versatile addition to the expanding tool chest of open-source miniscopes that will increase access to multi-region circuit investigations during unrestrained behavior.
UR - http://www.scopus.com/inward/record.url?scp=85078686981&partnerID=8YFLogxK
U2 - https://doi.org/10.7554/eLife.49987
DO - https://doi.org/10.7554/eLife.49987
M3 - Article
C2 - 31934857
SN - 2050-084X
VL - 9
JO - eLife
JF - eLife
M1 - e49987
ER -