TY - JOUR
T1 - Non-invasive quantification of the mitochondrial redox state in livers during machine perfusion
AU - de Vries, Reinier J.
AU - Cronin, Stephanie E. J.
AU - Romfh, Padraic
AU - Pendexter, Casie A.
AU - Jain, Rohil
AU - Wilks, Benjamin T.
AU - Raigani, Siavash
AU - van Gulik, Thomas M.
AU - Chen, Peili
AU - Yeh, Heidi
AU - Uygun, Korkut
AU - Tessier, Shannon N.
N1 - Publisher Copyright: © 2021 de Vries et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2021/10/1
Y1 - 2021/10/1
N2 - Ischemia reperfusion injury (IRI) is a critical problem in liver transplantation that can lead to life-threatening complications and substantially limit the utilization of livers for transplantation. However, because there are no early diagnostics available, fulminant injury may only become evident post-transplant. Mitochondria play a central role in IRI and are an ideal diagnostic target. During ischemia, changes in the mitochondrial redox state form the first link in the chain of events that lead to IRI. In this study we used resonance Raman spectroscopy to provide a rapid, non-invasive, and label-free diagnostic for quantification of the hepatic mitochondrial redox status. We show this diagnostic can be used to significantly distinguish transplantable versus non-transplantable ischemically injured rat livers during oxygenated machine perfusion and demonstrate spatial differences in the response of mitochondrial redox to ischemia reperfusion. This novel diagnostic may be used in the future to predict the viability of human livers for transplantation and as a tool to better understand the mechanisms of hepatic IRI.
AB - Ischemia reperfusion injury (IRI) is a critical problem in liver transplantation that can lead to life-threatening complications and substantially limit the utilization of livers for transplantation. However, because there are no early diagnostics available, fulminant injury may only become evident post-transplant. Mitochondria play a central role in IRI and are an ideal diagnostic target. During ischemia, changes in the mitochondrial redox state form the first link in the chain of events that lead to IRI. In this study we used resonance Raman spectroscopy to provide a rapid, non-invasive, and label-free diagnostic for quantification of the hepatic mitochondrial redox status. We show this diagnostic can be used to significantly distinguish transplantable versus non-transplantable ischemically injured rat livers during oxygenated machine perfusion and demonstrate spatial differences in the response of mitochondrial redox to ischemia reperfusion. This novel diagnostic may be used in the future to predict the viability of human livers for transplantation and as a tool to better understand the mechanisms of hepatic IRI.
UR - http://www.scopus.com/inward/record.url?scp=85118247907&partnerID=8YFLogxK
U2 - https://doi.org/10.1371/journal.pone.0258833
DO - https://doi.org/10.1371/journal.pone.0258833
M3 - Article
C2 - 34705828
SN - 1932-6203
VL - 16
JO - PLOS ONE
JF - PLOS ONE
IS - 10 October
M1 - e0258833
ER -