One naive T cell, multiple fates in CD8+ T cell differentiation

Carmen Gerlach, Jeroen W. J. van Heijst, Erwin Swart, Daoud Sie, Nicola Armstrong, Ron M. Kerkhoven, Dietmar Zehn, Michael J. Bevan, Koen Schepers, Ton N. M. Schumacher

Research output: Contribution to journalArticleAcademicpeer-review

146 Citations (Scopus)

Abstract

The mechanism by which the immune system produces effector and memory T cells is largely unclear. To allow a large-scale assessment of the development of single naive T cells into different subsets, we have developed a technology that introduces unique genetic tags (barcodes) into naive T cells. By comparing the barcodes present in antigen-specific effector and memory T cell populations in systemic and local infection models, at different anatomical sites, and for TCR-pMHC interactions of different avidities, we demonstrate that under all conditions tested, individual naive T cells yield both effector and memory CD8+ T cell progeny. This indicates that effector and memory fate decisions are not determined by the nature of the priming antigen-presenting cell or the time of T cell priming. Instead, for both low and high avidity T cells, individual naive T cells have multiple fates and can differentiate into effector and memory T cell subsets
Original languageEnglish
Pages (from-to)1235-1246
Number of pages12
JournalJournal of Experimental Medicine
Volume207
Issue number6
DOIs
Publication statusPublished - 7 Jun 2010

Keywords

  • Animals
  • CD8-Positive T-Lymphocytes/cytology
  • Cell Differentiation/immunology
  • Cell Lineage/immunology
  • Immunologic Memory/immunology
  • Listeriosis/complications
  • Lymphoid Tissue/cytology
  • Mice
  • Mice, Inbred C57BL
  • Orthomyxoviridae Infections/complications
  • Receptors, Antigen, T-Cell/immunology
  • T-Lymphocyte Subsets/cytology

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