TY - JOUR
T1 - Online monitoring of myocardial bioprosthesis for cardiac repair
AU - Prat-Vidal, Cristina
AU - Gálvez-Montón, Carolina
AU - Puig-Sanvicens, Verónica
AU - Sanchez, Benjamin
AU - Díaz-Güemes, Idoia
AU - Bogónez-Franco, Paco
AU - Perea-Gil, Isaac
AU - Casas-Solà, Anna
AU - Roura, Santiago
AU - Llucià-Valldeperas, Aida
AU - Soler-Botija, Carolina
AU - Sánchez-Margallo, Francisco M
AU - Semino, Carlos E
AU - Bragos, Ramon
AU - Bayes-Genis, Antoni
PY - 2014/7/1
Y1 - 2014/7/1
N2 - BACKGROUND/OBJECTIVES: The aim of this study was to develop a myocardial bioprosthesis for cardiac repair with an integrated online monitoring system. Myocardial infarction (MI) causes irreversible myocyte loss and scar formation. Tissue engineering to reduce myocardial scar size has been tested with variable success, yet scar formation and modulation by an engineered graft is incompletely characterized.METHODS: Decellularized human pericardium was embedded using self-assembling peptide RAD16-I with or without GFP-labeled mediastinal adipose tissue-derived progenitor cells (MATPCs). Resulting bioprostheses were implanted over the ischemic myocardium in the swine model of MI (n=8 treated and n=5 control animals). For in vivo electrical impedance spectroscopy (EIS) monitoring, two electrodes were anchored to construct edges, covered by NanoGold particles and connected to an impedance-based implantable device. Histological evaluation was performed to identify and characterize GFP cells on post mortem myocardial sections.RESULTS: Pluripotency, cardiomyogenic and endothelial potential and migratory capacity of porcine-derived MATPCs were demonstrated in vitro. Decellularization protocol efficiency, biodegradability, as well as in vitro biocompatibility after recellularization were also verified. One month after myocardial bioprosthesis implantation, morphometry revealed a 36% reduction in infarct area, Ki67(+)-GFP(+)-MATPCs were found at infarct core and border zones, and bioprosthesis vascularization was confirmed by presence of Griffonia simplicifolia lectin I (GSLI) B4 isolectin(+)-GFP(+)-MATPCs. Electrical impedance measurement at low and high frequencies (10 kHz-100 kHz) allowed online monitoring of scar maturation.CONCLUSIONS: With clinical translation as ultimate goal, this myocardial bioprosthesis holds promise to be a viable candidate for human cardiac repair.
AB - BACKGROUND/OBJECTIVES: The aim of this study was to develop a myocardial bioprosthesis for cardiac repair with an integrated online monitoring system. Myocardial infarction (MI) causes irreversible myocyte loss and scar formation. Tissue engineering to reduce myocardial scar size has been tested with variable success, yet scar formation and modulation by an engineered graft is incompletely characterized.METHODS: Decellularized human pericardium was embedded using self-assembling peptide RAD16-I with or without GFP-labeled mediastinal adipose tissue-derived progenitor cells (MATPCs). Resulting bioprostheses were implanted over the ischemic myocardium in the swine model of MI (n=8 treated and n=5 control animals). For in vivo electrical impedance spectroscopy (EIS) monitoring, two electrodes were anchored to construct edges, covered by NanoGold particles and connected to an impedance-based implantable device. Histological evaluation was performed to identify and characterize GFP cells on post mortem myocardial sections.RESULTS: Pluripotency, cardiomyogenic and endothelial potential and migratory capacity of porcine-derived MATPCs were demonstrated in vitro. Decellularization protocol efficiency, biodegradability, as well as in vitro biocompatibility after recellularization were also verified. One month after myocardial bioprosthesis implantation, morphometry revealed a 36% reduction in infarct area, Ki67(+)-GFP(+)-MATPCs were found at infarct core and border zones, and bioprosthesis vascularization was confirmed by presence of Griffonia simplicifolia lectin I (GSLI) B4 isolectin(+)-GFP(+)-MATPCs. Electrical impedance measurement at low and high frequencies (10 kHz-100 kHz) allowed online monitoring of scar maturation.CONCLUSIONS: With clinical translation as ultimate goal, this myocardial bioprosthesis holds promise to be a viable candidate for human cardiac repair.
KW - Adipose Tissue/cytology
KW - Aged
KW - Animals
KW - Bioprosthesis
KW - Cardiac Surgical Procedures/methods
KW - Cells, Cultured
KW - Female
KW - Humans
KW - Male
KW - Middle Aged
KW - Monitoring, Ambulatory/methods
KW - Myocardial Ischemia/pathology
KW - Online Systems
KW - Pericardium/cytology
KW - Prosthesis Implantation/methods
KW - Swine
U2 - https://doi.org/10.1016/j.ijcard.2014.04.181
DO - https://doi.org/10.1016/j.ijcard.2014.04.181
M3 - Article
C2 - 24820760
SN - 0167-5273
VL - 174
SP - 654
EP - 661
JO - International journal of cardiology
JF - International journal of cardiology
IS - 3
ER -