TY - JOUR
T1 - Organotypic-liver slide culture systems to explore the role of extracellular vesicles in pancreatic cancer metastatic behavior and guide new therapeutic approaches
AU - Supadmanaba, I. Gede Putu
AU - Comandatore, Annalisa
AU - Morelli, Luca
AU - Giovannetti, Elisa
AU - Lagerweij, Tonny
N1 - Funding Information: This work was supported by the CCA Foundation 2015 and 2018 grants (E.G.); KWF Dutch Cancer Society grants #11957/13598 (E.G.); AIRC/Start-Up and IG-24444 grant (E.G.); Indonesian Endowment Fund Scholarship (I.G.P.S.) and Rotary Global Grant GG2123321 (A.C.) The authors would like to thank Tim Heistek (CNCR. AmsterdamUMC) for assisting with the vibratome experiments. Publisher Copyright: © 2021 Informa UK Limited, trading as Taylor & Francis Group. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021
Y1 - 2021
N2 - Introduction: Recent studies suggested that extracellular vesicles (EVs) play a role both in the metastatic niche formation and in the progression of several tumors, including pancreatic cancer. In particular, the effects of EVs on metastasis should be studied in model systems that take into account both the tumor cells and the metastatic site/tumor microenvironment. Studies with labeled EVs or EV-secreting cells in ex vivo models will reflect the physiological and pathological functions of EVs. The organotypic-tissue slide culture systems can fulfill such a role. Areas covered: This review provides an overview of available organotypic-culture slide systems. We specifically focus on the assay system of liver culture-slides in combination with pancreatic tumors, which can be modulated to test the efficacy of new therapeutic approaches. Expert opinion: The intercellular exchange of EVs has emerged as a biologically relevant phenomenon to drive cancer metastasis. However, further models need to be developed to better elucidate the functional roles of EVs. The use of novel organotypic slide culture systems provides the opportunity to explore the role of EVs in the metastatic behavior of pancreatic cancer, decreasing the use of costly and cumbersome organoid or animal models.
AB - Introduction: Recent studies suggested that extracellular vesicles (EVs) play a role both in the metastatic niche formation and in the progression of several tumors, including pancreatic cancer. In particular, the effects of EVs on metastasis should be studied in model systems that take into account both the tumor cells and the metastatic site/tumor microenvironment. Studies with labeled EVs or EV-secreting cells in ex vivo models will reflect the physiological and pathological functions of EVs. The organotypic-tissue slide culture systems can fulfill such a role. Areas covered: This review provides an overview of available organotypic-culture slide systems. We specifically focus on the assay system of liver culture-slides in combination with pancreatic tumors, which can be modulated to test the efficacy of new therapeutic approaches. Expert opinion: The intercellular exchange of EVs has emerged as a biologically relevant phenomenon to drive cancer metastasis. However, further models need to be developed to better elucidate the functional roles of EVs. The use of novel organotypic slide culture systems provides the opportunity to explore the role of EVs in the metastatic behavior of pancreatic cancer, decreasing the use of costly and cumbersome organoid or animal models.
KW - Extracellular vesicles
KW - metastasis
KW - organotypic-tissue slide culture systems
KW - pancreatic cancer
KW - preclinical models
KW - tumor microenvironment
UR - http://www.scopus.com/inward/record.url?scp=85106310746&partnerID=8YFLogxK
U2 - https://doi.org/10.1080/17425255.2021.1925646
DO - https://doi.org/10.1080/17425255.2021.1925646
M3 - Article
C2 - 33945374
SN - 1742-5255
VL - 17
SP - 937
EP - 946
JO - Expert Opinion on Drug Metabolism & Toxicology
JF - Expert Opinion on Drug Metabolism & Toxicology
IS - 8
ER -