TY - JOUR
T1 - Plasma concentrations of extracellular vesicles are decreased in patients with post-infarct cardiac remodelling
AU - Gąsecka, Aleksandra
AU - Pluta, Kinga
AU - Solarska, Katarzyna
AU - Rydz, Bartłomiej
AU - Eyileten, Ceren
AU - Postula, Marek
AU - van der Pol, Edwin
AU - Nieuwland, Rienk
AU - Budnik, Monika
AU - Kochanowski, Janusz
AU - Jaguszewski, Miłosz J.
AU - Szarpak, Łukasz
AU - Mazurek, Tomasz
AU - Kapłon-Cieślicka, Agnieszka
AU - Opolski, Grzegorz
AU - Filipiak, Krzysztof J.
N1 - Funding Information: Funding: The work was funded by the PRELUDIUM Grant of the Polish National Science Centre (2018/31/N/NZ7/02260) to A. Gąsecka, PRELUDIUM Grant of the Polish National Science Centre (2017/25/N/NZ5/00545) to C. Eyileten and VENI grant to E. van der Pol (15924). The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/2/1
Y1 - 2021/2/1
N2 - Background, the mechanisms underlying left ventricular remodelling (LVR) after acute myocardial infarction (AMI) remain obscure. In the course of AMI, blood cells and endothelial cells release extracellular vesicles (EVs). We hypothesized that changes in EV concentrations after AMI may underlie LVR. Methods, plasma concentrations of EVs from endothelial cells (CD146+), erythrocytes (CD235a+), leukocytes (CD45+), platelets (CD61+), activated platelets (P-selectin+), and EVs exposing phosphatidylserine after AMI were determined by flow cytometry in 55 patients with the first AMI. LVR was defined as an increase in left ventricular end-diastolic volume by 20% at 6 months after AMI, compared to baseline. Results, baseline concentrations of EVs from endothelial cells, erythrocytes and platelets were lower in patients who developed LVR (p ≤ 0.02 for all). Concentrations of EVs from endothelial cells and erythrocytes were independent LVR predictors (OR 8.2, CI 1.3–54.2 and OR 17.8, CI 2.3–138.6, respectively) in multivariate analysis. Combining the three EV subtypes allowed to predict LVR with 83% sensitivity and 87% specificity. Conclusions, decreased plasma concentrations of EVs from endothelial cells, erythrocytes and platelets predict LVR after AMI. Since EV release EVs contributes to cellular homeostasis by waste removal, decreased concentrations of EVs may indicate dysfunctional cardiac homeostasis after AMI, thus promoting LVR.
AB - Background, the mechanisms underlying left ventricular remodelling (LVR) after acute myocardial infarction (AMI) remain obscure. In the course of AMI, blood cells and endothelial cells release extracellular vesicles (EVs). We hypothesized that changes in EV concentrations after AMI may underlie LVR. Methods, plasma concentrations of EVs from endothelial cells (CD146+), erythrocytes (CD235a+), leukocytes (CD45+), platelets (CD61+), activated platelets (P-selectin+), and EVs exposing phosphatidylserine after AMI were determined by flow cytometry in 55 patients with the first AMI. LVR was defined as an increase in left ventricular end-diastolic volume by 20% at 6 months after AMI, compared to baseline. Results, baseline concentrations of EVs from endothelial cells, erythrocytes and platelets were lower in patients who developed LVR (p ≤ 0.02 for all). Concentrations of EVs from endothelial cells and erythrocytes were independent LVR predictors (OR 8.2, CI 1.3–54.2 and OR 17.8, CI 2.3–138.6, respectively) in multivariate analysis. Combining the three EV subtypes allowed to predict LVR with 83% sensitivity and 87% specificity. Conclusions, decreased plasma concentrations of EVs from endothelial cells, erythrocytes and platelets predict LVR after AMI. Since EV release EVs contributes to cellular homeostasis by waste removal, decreased concentrations of EVs may indicate dysfunctional cardiac homeostasis after AMI, thus promoting LVR.
KW - Acute myocardial infarction
KW - Extracellular vesicles
KW - Flow cytometry
KW - Heart failure
KW - Left-ventricular remodelling
UR - http://www.scopus.com/inward/record.url?scp=85100808918&partnerID=8YFLogxK
U2 - https://doi.org/10.3390/biology10020097
DO - https://doi.org/10.3390/biology10020097
M3 - Article
C2 - 33573196
SN - 2079-7737
VL - 10
SP - 1
EP - 13
JO - Biology
JF - Biology
IS - 2
M1 - 97
ER -