TY - JOUR
T1 - Plasma lipid profiles discriminate bacterial from viral infection in febrile children
AU - EUCLIDS Consortium
AU - Wang, Xinzhu
AU - Nijman, Ruud
AU - Camuzeaux, Stephane
AU - Sands, Caroline
AU - Jackson, Heather
AU - Kaforou, Myrsini
AU - Emonts, Marieke
AU - Herberg, Jethro A.
AU - Maconochie, Ian
AU - Carrol, Enitan D.
AU - Paulus, Stephane C.
AU - Zenz, Werner
AU - van der Flier, Michiel
AU - de Groot, Ronald
AU - Martinon-Torres, Federico
AU - Schlapbach, Luregn J.
AU - Pollard, Andrew J.
AU - Fink, Colin
AU - Kuijpers, Taco T.
AU - Anderson, Suzanne
AU - Lewis, Matthew R.
AU - Levin, Michael
AU - McClure, Myra
AU - Gormley, Stuart
AU - Hamilton, Shea
AU - Hourmat, Bernardo
AU - Hoggart, Clive
AU - Sancho-Shimizu, Vanessa
AU - Wright, Victoria
AU - Abdulla, Amina
AU - Agapow, Paul
AU - Bartlett, Maeve
AU - Bellos, Evangelos
AU - Eleftherohorinou, Hariklia
AU - Galassini, Rachel
AU - Inwald, David
AU - Mashbat, Meg
AU - Menikou, Stefanie
AU - Mustafa, Sobia
AU - Nadel, Simon
AU - van Furth, A. M. Tutu
AU - Pajkrt, D.
AU - Sanders, E. A. M.
AU - de Vries, E.
AU - Donker, A. E.
AU - Jacobs, M. A. M.
AU - Miedema, C. J.
AU - Obihara, C. C.
AU - van der Meer, H.
AU - van Wermeskerken, A. M.
AU - Rahman, Rahmeen
AU - Thakker, Clare
AU - Coin, Lachlan M. J.
AU - Bokhandi, S.
AU - Power, Sue
AU - Barham, Heather
AU - Pathan, Dr N.
AU - van de Beek, D.
AU - van der Ende, A.
PY - 2019/12/1
Y1 - 2019/12/1
N2 - Fever is the most common reason that children present to Emergency Departments. Clinical signs and symptoms suggestive of bacterial infection are often non-specific, and there is no definitive test for the accurate diagnosis of infection. The ‘omics’ approaches to identifying biomarkers from the host-response to bacterial infection are promising. In this study, lipidomic analysis was carried out with plasma samples obtained from febrile children with confirmed bacterial infection (n = 20) and confirmed viral infection (n = 20). We show for the first time that bacterial and viral infection produces distinct profile in the host lipidome. Some species of glycerophosphoinositol, sphingomyelin, lysophosphatidylcholine and cholesterol sulfate were higher in the confirmed virus infected group, while some species of fatty acids, glycerophosphocholine, glycerophosphoserine, lactosylceramide and bilirubin were lower in the confirmed virus infected group when compared with confirmed bacterial infected group. A combination of three lipids achieved an area under the receiver operating characteristic (ROC) curve of 0.911 (95% CI 0.81 to 0.98). This pilot study demonstrates the potential of metabolic biomarkers to assist clinicians in distinguishing bacterial from viral infection in febrile children, to facilitate effective clinical management and to the limit inappropriate use of antibiotics.
AB - Fever is the most common reason that children present to Emergency Departments. Clinical signs and symptoms suggestive of bacterial infection are often non-specific, and there is no definitive test for the accurate diagnosis of infection. The ‘omics’ approaches to identifying biomarkers from the host-response to bacterial infection are promising. In this study, lipidomic analysis was carried out with plasma samples obtained from febrile children with confirmed bacterial infection (n = 20) and confirmed viral infection (n = 20). We show for the first time that bacterial and viral infection produces distinct profile in the host lipidome. Some species of glycerophosphoinositol, sphingomyelin, lysophosphatidylcholine and cholesterol sulfate were higher in the confirmed virus infected group, while some species of fatty acids, glycerophosphocholine, glycerophosphoserine, lactosylceramide and bilirubin were lower in the confirmed virus infected group when compared with confirmed bacterial infected group. A combination of three lipids achieved an area under the receiver operating characteristic (ROC) curve of 0.911 (95% CI 0.81 to 0.98). This pilot study demonstrates the potential of metabolic biomarkers to assist clinicians in distinguishing bacterial from viral infection in febrile children, to facilitate effective clinical management and to the limit inappropriate use of antibiotics.
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85075710136&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/31776453
U2 - https://doi.org/10.1038/s41598-019-53721-1
DO - https://doi.org/10.1038/s41598-019-53721-1
M3 - Article
C2 - 31776453
SN - 2045-2322
VL - 9
JO - Scientific reports
JF - Scientific reports
IS - 1
M1 - 17714
ER -