Plasmodium falciparum sexual conversion rates can be affected by artemisinin-based treatment in naturally infected malaria patients

Harvie P. Portugaliza, H. Magloire Natama, Pieter Guetens, Eduard Rovira-Vallbona, Athanase M. Somé, Aida Millogo, D. Florence Ouédraogo, Innocent Valéa, Hermann Sorgho, Halidou Tinto, Nguyen van Hong, Antonio Sitoe, Rosauro Varo, Quique Bassat, Alfred Cortés, Anna Rosanas-Urgell

Research output: Contribution to JournalArticleAcademicpeer-review

Abstract

Background: Artemisinins (ART) are the key component of the frontline antimalarial treatment, but their impact on Plasmodium falciparum sexual conversion rates in natural malaria infections remains unknown. This is an important knowledge gap because sexual conversion rates determine the relative parasite investment between maintaining infection in the same human host and transmission to mosquitoes. Methods: The primary outcome of this study was to assess the impact of ART-based treatment on sexual conversion rates by comparing the relative transcript levels of pfap2-g and other sexual ring biomarkers (SRBs) before and after treatment. We analysed samples from previously existing cohorts in Vietnam, Burkina Faso and Mozambique (in total, n=109) collected before treatment and at 12 h intervals after treatment. As a secondary objective, we investigated factors that may influence the effect of treatment on sexual conversion rates. Findings: In the majority of infections from the African cohorts, but not from Vietnam, we observed increased expression of pfap2-g and other SRBs after treatment. Estimated parasite age at the time of treatment was negatively correlated with the increase in pfap2-g transcript levels, suggesting that younger parasites are less susceptible to stimulation of sexual conversion. Interpretation: We observed enhanced expression of SRBs after ART-based treatment in many patients, which suggests that in natural malaria infections sexual conversion rates can be altered by treatment. ART-based treatment reduces the potential of a treated individual to transmit the disease, but we hypothesise that under some circumstances this reduction may be attenuated by ART-enhanced sexual conversion. Funding: Spanish Agencia Estatal de Investigación (AEI), European Regional Development Fund (ERDF, European Union), Belgium Development Cooperation (DGD), Canadian University Health Network (UHN), TransGlobalHealth–Erasmus Mundus (European Union).
Original languageEnglish
Article number104198
JournalEBIOMEDICINE
Volume83
DOIs
Publication statusPublished - 1 Sep 2022

Keywords

  • Artemisinin
  • Malaria transmission
  • Plasmodium falciparum
  • Sexual conversion
  • pfap2-g

Cite this