Abstract
With the trend of movingmolecular tests from clinical diagnosticlaboratories to on-site testing, there is a need for nucleic acid based diagnostic tools combining the sensitivity, specificity and flexibility of established geneticdiagnostics with the ease, cost effectiveness and speed of isothermal amplification and detectionmethods. A promising new nucleic acid detectionmethod is Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated nuclease (Cas)-based sensing. In this method Cas effector proteins are used as highly specific sequence recognition elements that can be combined with many different read-out methods for on-site point-of-care testing. This review covers the technical aspects of integrating CRISPR/Cas technology in miniaturized sensors for analysis on-site. We start with a short introduction to CRISPR/Cas systems and the different effector proteins and continue with reviewing the recent developments of integrating CRISPR sensing in miniaturized sensors for point-of-care applications. Finally, we discuss the challenges of point-of-care CRISPR sensing and describe future research perspectives.
Original language | English |
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Article number | 112445 |
Pages (from-to) | 112445 |
Journal | Biosensors and Bioelectronics |
Volume | 166 |
DOIs | |
Publication status | Published - 15 Oct 2020 |
Keywords
- CRISPR sensing
- CRISPR/Cas
- DNA sensing
- Diagnosis
- Genomic diagnostic tools
- Point-of-care