Polar metabolomics in human muscle biopsies using a liquid-liquid extraction and full-scan LC-MS

Bauke V. Schomakers; Jill Hermans; Yorrick R. J. Jaspers; Gajja Salomons; Frédéric M. Vaz; Michel van Weeghel; Riekelt H. Houtkooper

doi:https://doi.org/10.1016/j.xpro.2022.101302

Polar metabolomics in human muscle biopsies using a liquid-liquid extraction and full-scan LC-MS

Bauke V. Schomakers, Jill Hermans, Yorrick R. J. Jaspers, Gajja Salomons, Frédéric M. Vaz, Michel van Weeghel, Riekelt H. Houtkooper

Research output: Contribution to journal › Article › Academic › peer-review

12 Citations (Scopus)

Abstract

We describe here a user-friendly analysis protocol for semi-targeted polar metabolomics in human muscle biopsies using Zwitterionic Hydrophilic Interaction Liquid Chromatography and high-resolution full-scan mass spectrometry. Previously, this protocol has been used for Caenorhabditis elegans. Here we show that it can be successfully applied to human muscle biopsies with minor adjustments. Summarized instructions for other matrices are also provided. As peak integration in metabolomics can be challenging, we provide expected retention times and extensive peak descriptions to aid this process. For complete details on the use and execution of this protocol, please refer to Molenaars et al. (2021).

Original language	English
Article number	101302
Journal	STAR Protocols
Volume	3
Issue number	2
DOIs	https://doi.org/10.1016/j.xpro.2022.101302
Publication status	Published - 17 Jun 2022

Keywords

Health Sciences
Mass Spectrometry
Metabolism
Metabolomics

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https://doi.org/10.1016/j.xpro.2022.101302

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title = "Polar metabolomics in human muscle biopsies using a liquid-liquid extraction and full-scan LC-MS",

abstract = "We describe here a user-friendly analysis protocol for semi-targeted polar metabolomics in human muscle biopsies using Zwitterionic Hydrophilic Interaction Liquid Chromatography and high-resolution full-scan mass spectrometry. Previously, this protocol has been used for Caenorhabditis elegans. Here we show that it can be successfully applied to human muscle biopsies with minor adjustments. Summarized instructions for other matrices are also provided. As peak integration in metabolomics can be challenging, we provide expected retention times and extensive peak descriptions to aid this process. For complete details on the use and execution of this protocol, please refer to Molenaars et al. (2021).",

keywords = "Health Sciences, Mass Spectrometry, Metabolism, Metabolomics",

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AU - Schomakers, Bauke V.

AU - Hermans, Jill

AU - Jaspers, Yorrick R. J.

AU - Salomons, Gajja

AU - Vaz, Frédéric M.

AU - van Weeghel, Michel

AU - Houtkooper, Riekelt H.

N1 - Funding Information: Work in the Houtkooper group is financially supported by a grant from the Velux Stiftung (no. 1063 ). The graphical abstract was made with BioRender. Publisher Copyright: © 2022 The Author(s)

PY - 2022/6/17

Y1 - 2022/6/17

N2 - We describe here a user-friendly analysis protocol for semi-targeted polar metabolomics in human muscle biopsies using Zwitterionic Hydrophilic Interaction Liquid Chromatography and high-resolution full-scan mass spectrometry. Previously, this protocol has been used for Caenorhabditis elegans. Here we show that it can be successfully applied to human muscle biopsies with minor adjustments. Summarized instructions for other matrices are also provided. As peak integration in metabolomics can be challenging, we provide expected retention times and extensive peak descriptions to aid this process. For complete details on the use and execution of this protocol, please refer to Molenaars et al. (2021).

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KW - Health Sciences

KW - Mass Spectrometry

KW - Metabolism

KW - Metabolomics

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Polar metabolomics in human muscle biopsies using a liquid-liquid extraction and full-scan LC-MS

Abstract

Keywords

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