TY - JOUR
T1 - Pool size estimations for dense-core vesicles in mammalian CNS neurons
AU - Persoon, Claudia M.
AU - Moro, Alessandro
AU - Nassal, Joris P.
AU - Farina, Margherita
AU - Broeke, Jurjen H.
AU - Arora, Swati
AU - Dominguez, Natalia
AU - van Weering, Jan R. T.
AU - Toonen, Ruud F.
AU - Verhage, Matthijs
PY - 2018/10/15
Y1 - 2018/10/15
N2 - Neuropeptides are essential signaling molecules transported and secreted by dense-core vesicles (DCVs), but the number of DCVs available for secretion, their subcellular distribution, and release probability are unknown. Here, we quantified DCV pool sizes in three types of mammalian CNS neurons in vitro and in vivo. Super-resolution and electron microscopy reveal a total pool of 1,400–18,000 DCVs, correlating with neurite length. Excitatory hippocampal and inhibitory striatal neurons in vitro have a similar DCV density, and thalamo-cortical axons in vivo have a slightly higher density. Synapses contain on average two to three DCVs, at the periphery of synaptic vesicle clusters. DCVs distribute equally in axons and dendrites, but the vast majority (80%) of DCV fusion events occur at axons. The release probability of DCVs is 1–6%, depending on the stimulation. Thus, mammalian CNS neurons contain a large pool of DCVs of which only a small fraction can fuse, preferentially at axons.
AB - Neuropeptides are essential signaling molecules transported and secreted by dense-core vesicles (DCVs), but the number of DCVs available for secretion, their subcellular distribution, and release probability are unknown. Here, we quantified DCV pool sizes in three types of mammalian CNS neurons in vitro and in vivo. Super-resolution and electron microscopy reveal a total pool of 1,400–18,000 DCVs, correlating with neurite length. Excitatory hippocampal and inhibitory striatal neurons in vitro have a similar DCV density, and thalamo-cortical axons in vivo have a slightly higher density. Synapses contain on average two to three DCVs, at the periphery of synaptic vesicle clusters. DCVs distribute equally in axons and dendrites, but the vast majority (80%) of DCV fusion events occur at axons. The release probability of DCVs is 1–6%, depending on the stimulation. Thus, mammalian CNS neurons contain a large pool of DCVs of which only a small fraction can fuse, preferentially at axons.
KW - dense-core vesicles
KW - pool sizes
KW - release probability
KW - secretion
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85052960497&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/30185408
UR - http://www.scopus.com/inward/record.url?scp=85052960497&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85052960497&partnerID=8YFLogxK
UR - http://europepmc.org/articles/PMC6187028
U2 - https://doi.org/10.15252/embj.201899672
DO - https://doi.org/10.15252/embj.201899672
M3 - Article
C2 - 30185408
SN - 0261-4189
VL - 37
SP - 1
EP - 18
JO - EMBO Journal
JF - EMBO Journal
IS - 20
M1 - e99672
ER -