@article{a089b1668d6f4d1886b536f27949fdca,
title = "Pre-analytical sample handling effects on blood cytokine levels: Quality control of a COVID-19 biobank",
abstract = "Aim: We investigated the effect of pre-analytical sample handling variations on coronavirus disease 2019-relevant circulating cytokine levels IFN-γ, IL-10, IL-12p70, IL-17A, IL-6 and TNF-α. Materials & methods: We collected blood in different collection tubes (ethylenediaminetetraacetic acid, sodium citrate, lithium heparin, serum), and subjected ethylenediaminetetraacetic acid plasma to among others increasing delays in centrifugation or -80°C storage. Six subjects were included in each experimental condition. Cytokine levels were measured in these samples using the Simoa Cytokine 6-plex kit. Results: Different tube types resulted in different blood cytokine levels. IL-17A and IL-6 levels declined with 3 h centrifugation delay. IFN-γlevels declined with 24 h postcentrifugation storage delay. IL-17A levels declined with 2-week storage delay. Conclusion: It is recommended to centrifuge tubes quickly following collection, for accurate cytokine measurement.",
keywords = "Biobank, interferon, interleukin, pre-analytics, stability, tumor necrosis factor",
author = "Verberk, {Inge Mw} and Nossent, {Esther J.} and Bontkes, {Hetty J.} and Teunissen, {Charlotte E.}",
note = "Funding Information: IMW Verberk, EJ Nossent and HJ Bontkes report no disclosures. CE Teunissen has a collaboration contract with ADx Neurosciences and Quanterix, and performed contract research for or received grants from AxonNeurosciences, Biogen, Boehringer, Brainstorm Therapeutics, Celgene, EIP Pharma, Esai, Janssen prevention center, Roche, Toyama, Vivoryon. Funding sources had no role in study design, data collection, analysis or interpretation or in writing the report. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript. Publisher Copyright: {\textcopyright} 2021 Future Medicine Ltd.. All rights reserved. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.",
year = "2021",
month = aug,
day = "1",
doi = "https://doi.org/10.2217/bmm-2020-0770",
language = "English",
volume = "15",
pages = "987--997",
journal = "Biomarkers in medicine",
issn = "1752-0363",
publisher = "Future Medicine Ltd.",
number = "12",
}