TY - JOUR
T1 - Preclinical Detection of Non-catheter Related Late-onset Sepsis in Preterm Infants by Fecal Volatile Compounds Analysis
T2 - A Prospective, Multi-center Cohort Study
AU - Berkhout, Daniel J.C.
AU - Niemarkt, Hendrik J.
AU - Andriessen, Peter
AU - Vijlbrief, Daniel C.
AU - Bomers, Marije K.
AU - Cossey, Veerle
AU - Hulzebos, Christian V.
AU - Van Kaam, Anton H.
AU - Kramer, Boris W.
AU - Van Lingen, Richard A.
AU - Wicaksono, Alfian N.
AU - Covington, James A.
AU - Van Weissenbruch, Mirjam M.
AU - Benninga, Marc A.
AU - De Boer, Nanne K.H.
AU - De Meij, Tim G.J.
N1 - Publisher Copyright: © 2020 Lippincott Williams and Wilkins. All rights reserved. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/4/1
Y1 - 2020/4/1
N2 - Background : Late onset sepsis (LOS) in preterm infants is preceded by fecal volatile organic compound (VOC) alterations, suggesting an etiologic role of gut microbiota in LOS rather than being primarily caused by central venous catheters (CVC). To increase our knowledge about the involvement of the gut microbiota in LOS, we analyzed fecal samples from septic infants without a CVC. Methods: In this prospective multicenter study, fecal samples were collected daily from all infants born at ≤30 weeks gestation. Fecal VOC profiles up to 3 days prior to sepsis onset from infants with non-catheter-related LOS were compared with profiles from non-septic controls by means of High-Field Asymmetric Waveform Ion Mobility Spectrometry. Results: In total, 104 fecal samples were analyzed. Fecal VOC profiles allowed for discrimination between non-catheter-related LOS cases (n = 24) and matched controls (n = 25). Discriminative accuracy increased after focusing on center of origin (area under the curve, sensitivity, specificity; 0.95, 100%, 83%) and after focusing on LOS cases caused by Staphylococcus epidermidis (0.95, 100%, 78%), the most cultured pathogen (n = 11). Conclusions: Fecal VOC profiles of preterm LOS infants without a CVC differed from matched controls underlining the increasing notion that aberrations in gut microbiota composition and activity may play a role in LOS etiology.
AB - Background : Late onset sepsis (LOS) in preterm infants is preceded by fecal volatile organic compound (VOC) alterations, suggesting an etiologic role of gut microbiota in LOS rather than being primarily caused by central venous catheters (CVC). To increase our knowledge about the involvement of the gut microbiota in LOS, we analyzed fecal samples from septic infants without a CVC. Methods: In this prospective multicenter study, fecal samples were collected daily from all infants born at ≤30 weeks gestation. Fecal VOC profiles up to 3 days prior to sepsis onset from infants with non-catheter-related LOS were compared with profiles from non-septic controls by means of High-Field Asymmetric Waveform Ion Mobility Spectrometry. Results: In total, 104 fecal samples were analyzed. Fecal VOC profiles allowed for discrimination between non-catheter-related LOS cases (n = 24) and matched controls (n = 25). Discriminative accuracy increased after focusing on center of origin (area under the curve, sensitivity, specificity; 0.95, 100%, 83%) and after focusing on LOS cases caused by Staphylococcus epidermidis (0.95, 100%, 78%), the most cultured pathogen (n = 11). Conclusions: Fecal VOC profiles of preterm LOS infants without a CVC differed from matched controls underlining the increasing notion that aberrations in gut microbiota composition and activity may play a role in LOS etiology.
KW - electronic nose
KW - late-onset sepsis
KW - neonatology
KW - preterm
KW - volatile organic compounds
UR - http://www.scopus.com/inward/record.url?scp=85082147029&partnerID=8YFLogxK
U2 - https://doi.org/10.1097/INF.0000000000002589
DO - https://doi.org/10.1097/INF.0000000000002589
M3 - Article
C2 - 32032172
SN - 0891-3668
VL - 39
SP - 330
EP - 335
JO - Pediatric Infectious Disease Journal
JF - Pediatric Infectious Disease Journal
IS - 4
ER -