TY - JOUR
T1 - Presence of human papillomavirus in semen of healthy men is firmly associated with HPV infections of the penile epithelium
AU - Luttmer, Roosmarijn
AU - Dijkstra, Maaike G.
AU - Snijders, Peter J. F.
AU - Jordanova, Ekaterina S.
AU - King, Audrey J.
AU - Pronk, Divera T. M.
AU - Foresta, Carlo
AU - Garolla, Andrea
AU - Hompes, Peter G. A.
AU - Berkhof, Johannes
AU - Bleeker, Maaike C. G.
AU - Doorbar, John
AU - Heideman, Daniëlle A. M.
AU - Meijer, Chris J. L. M.
PY - 2015
Y1 - 2015
N2 - To study the source of human papillomavirus (HPV) in semen. Observational study (CCMO-NL3248800010). Academic hospital-based laboratory. Healthy male volunteers (n = 213). One penile scrape and three semen samples were obtained per participant for HPV-DNA testing by both GP5+/6+ polymerase chain reaction (PCR) and SPF10-PCR to detect moderate/high and low viral loads, respectively; flat penile lesions (FPL) were detected by penoscopy. HPV-DNA presence in semen and penile scrapes, and the presence of FPL. HPV-DNA at moderate/high viral loads (i.e., GP5+/6+ PCR-positive) was detected in ≥1 semen sample(s) in 27% of participants. Most men with moderate/high viral loads in the penile scrape also had moderate/high viral loads in semen (85%). Men with a HPV-negative penile scrape were very unlikely to have moderate/high viral loads in semen (3%). The presence of HPV in semen was associated with the presence of HPV in the penile scrape also on a genotype-specific level. Having FPL was a risk factor for HPV presence in semen. HPV-DNA presence in semen of healthy men is common and associated with HPV infections of the penile epithelium. HPV-DNA presence in semen may result from desquamation of HPV-infected penile cells
AB - To study the source of human papillomavirus (HPV) in semen. Observational study (CCMO-NL3248800010). Academic hospital-based laboratory. Healthy male volunteers (n = 213). One penile scrape and three semen samples were obtained per participant for HPV-DNA testing by both GP5+/6+ polymerase chain reaction (PCR) and SPF10-PCR to detect moderate/high and low viral loads, respectively; flat penile lesions (FPL) were detected by penoscopy. HPV-DNA presence in semen and penile scrapes, and the presence of FPL. HPV-DNA at moderate/high viral loads (i.e., GP5+/6+ PCR-positive) was detected in ≥1 semen sample(s) in 27% of participants. Most men with moderate/high viral loads in the penile scrape also had moderate/high viral loads in semen (85%). Men with a HPV-negative penile scrape were very unlikely to have moderate/high viral loads in semen (3%). The presence of HPV in semen was associated with the presence of HPV in the penile scrape also on a genotype-specific level. Having FPL was a risk factor for HPV presence in semen. HPV-DNA presence in semen of healthy men is common and associated with HPV infections of the penile epithelium. HPV-DNA presence in semen may result from desquamation of HPV-infected penile cells
U2 - https://doi.org/10.1016/j.fertnstert.2015.06.028
DO - https://doi.org/10.1016/j.fertnstert.2015.06.028
M3 - Article
C2 - 26211884
SN - 0015-0282
VL - 104
SP - 838-844.e8
JO - Fertility and Sterility
JF - Fertility and Sterility
IS - 4
ER -