Prevalence of Neutralising Antibodies to HCoV-NL63 in Healthy Adults in Australia

Sean A Lynch, Kanta Subbarao, Siddhartha Mahanty, Bridget E Barber, Eileen V Roulis, Lia van der Hoek, James S McCarthy, Kirsten M Spann

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3 Citations (Scopus)

Abstract

The COVID-19 pandemic has highlighted the importance of understanding the immune response to seasonal human coronavirus (HCoV) infections such as HCoV-NL63, how existing neutralising antibodies to HCoV may modulate responses to SARS-CoV-2 infection, and the utility of seasonal HCoV as human challenge models. Therefore, in this study we quantified HCoV-NL63 neutralising antibody titres in a healthy adult population using plasma from 100 blood donors in Australia. A microneutralisation assay was performed with plasma diluted from 1:10 to 1:160 and tested with the HCoV-NL63 Amsterdam-1 strain. Neutralising antibodies were detected in 71% of the plasma samples, with a median geometric mean titre of 14. This titre was similar to those reported in convalescent sera taken from individuals 3-7 months following asymptomatic SARS-CoV-2 infection, and 2-3 years post-infection from symptomatic SARS-CoV-1 patients. HCoV-NL63 neutralising antibody titres decreased with increasing age (R2 = 0.042, p = 0.038), but did not differ by sex. Overall, this study demonstrates that neutralising antibody to HCoV-NL63 is detectable in approximately 71% of the healthy adult population of Australia. Similar titres did not impede the use of another seasonal human coronavirus (HCoV-229E) in a human challenge model, thus, HCoV-NL63 may be useful as a human challenge model for more pathogenic coronaviruses.

Original languageEnglish
Article number1618
JournalViruses
Volume13
Issue number8
DOIs
Publication statusPublished - 16 Aug 2021

Keywords

  • HCoV-NL63
  • Healthy adults
  • Neutralising antibody titre

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