Prevention of experimental autoimmune encephalomyelitis in Lewis rats by a novel fungal source of gamma-linolenic acid

L S Harbige, N Yeatman, S Amor, M A Crawford

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35 Citations (Scopus)


The effects of oral administration of linoleic- and gamma-linolenic-acid-rich oils on the clinical and histopathological manifestations of experimental autoimmune encephalomyelitis (EAE) were investigated in Lewis rats 7 d post-inoculation. gamma-Linolenic-acid-rich fungal (Mucor javanicus) oil at 500 mg/kg body weight abrogated clinical and histological signs of EAE although at doses of 200 and 1000 mg/kg body weight it was only effective in delaying the onset of clinical disease. Linoleic-acid-rich safflower-seed (Carthamus tinctorius) oil at 500, 750 and 1000 mg/kg body weight decreased the severity of clinical EAE disease in a dose-dependent manner. The effects in healthy animals of orally administered gamma-linolenic-acid-rich fungal oil (500 mg/kg body weight) and linoleic-acid-rich safflower-seed oil (1000 mg/kg body weight) on splenic lymphocyte proliferative responses to the T-cell mitogen concanavalin-A (Con A), membrane fatty acid composition and lymphocyte sub-sets were also studied. Both treatments enhanced the T-cell proliferative response to Con A. There was no significant effect on the proportion of splenic CD8+ or CD4+ lymphocytes. Compositional studies on splenic phosphoglyceride fatty acids of oil-treated animals suggest the above responses were associated with increases in spleen dihomo-gamma-linolenic and arachidonic acids.

Original languageEnglish
Pages (from-to)701-15
Number of pages15
JournalBritish journal of nutrition
Issue number5
Publication statusPublished - Nov 1995


  • Administration, Oral
  • Animals
  • Cell Division
  • Concanavalin A
  • Encephalomyelitis, Autoimmune, Experimental
  • Guinea Pigs
  • Journal Article
  • Lipid Metabolism
  • Mitogens
  • Mucor
  • Rats
  • Rats, Inbred Lew
  • Research Support, Non-U.S. Gov't
  • Safflower Oil
  • Spleen
  • T-Lymphocytes
  • alpha-Linolenic Acid
  • gamma-Linolenic Acid

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