Production of diacylglycerol and arachidonic acid in peripheral blood mononuclear cells from patients with asthma and healthy controls

M. W. Dooper, A. Timmermans, R. Aalbers, E. J. Weersink, J. G. de Monchy, H. F. Kauffman

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Enhanced activities of peripheral blood cells are a common characteristic of patients with asthma. Here we tested whether this could be due to a dysfunction in one or more signal transduction systems. The production of 1,2-diacylglycerol (1,2-DAG) and arachidonic acid was compared in mononuclear blood cells from patients with asthma (n = 10) and healthy controls (n = 12). Using three different stimuli (concanavalin A, aluminium fluoride or the calcium ionophore A23187) no difference in the production of both 1,2-DAG and arachidonic acid could be found between patients and controls before allergen challenge. Concanavalin A-induced 1,2-DAG production could be inhibited completely in the presence of isoprenaline; concanavalin A-induced arachidonic acid production, partially. The inhibitory effect of adenylate cyclase activation on the production of 1,2-DAG and arachidonic acid was identical in patients and controls. Following allergen challenge, there was a tendency to an increased production of 1,2-DAG and arachidonic acid in controls, whereas in patients there was a tendency to a decreased production. Enhanced cellular activities found in patients with asthma are not caused by an intrinsic dysfunction in production of 1,2-DAG and arachidonic acid
Original languageEnglish
Pages (from-to)248-254
JournalAnnals of allergy, asthma & immunology
Issue number3
Publication statusPublished - 1995

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