Profiling of volatile organic compounds produced by clinical Aspergillus isolates using gas chromatography-mass spectrometry

M.G. Gerritsen; P. Brinkman; N. Escobar; L.D. Bos; K. de Heer; M. Meijer; H.-G. Janssen; H. de Cock; H.A.B. Wosten; C.E. Visser; M.H.J. van Oers; P.J. Sterk; H. A. B. Wösten

doi:https://doi.org/10.1093/mmy/myx035

Profiling of volatile organic compounds produced by clinical Aspergillus isolates using gas chromatography-mass spectrometry

M.G. Gerritsen, P. Brinkman, N. Escobar, L.D. Bos, K. de Heer, M. Meijer, H.-G. Janssen, H. de Cock, H.A.B. Wosten, C.E. Visser, M.H.J. van Oers, P.J. Sterk, H. A. B. Wösten

Research output: Contribution to journal › Article › Academic › peer-review

15 Citations (Scopus)

Abstract

Volatile organic compounds (VOCs) in exhaled breath may identify the presence of invasive pulmonary aspergillosis. We aimed to detect VOC profiles emitted by in vitro cultured, clinical Aspergillus isolates using gas chromatography–mass spectrometry (GC-MS). Three clinical Aspergillus isolates and a reference strain were cultured while conidiation was prevented. Headspace samples were analyzed using a standardized method. Breath samples of patients from which the cultures were obtained were checked for the presence of the VOCs found in vitro. Each Aspergillus isolate produced a distinct VOC profile. These profiles could not be confirmed in exhaled breath in vivo.

Original language	English
Pages (from-to)	253-256
Number of pages	4
Journal	Medical mycology
Volume	56
Issue number	2
Early online date	2017
DOIs	https://doi.org/10.1093/mmy/myx035
Publication status	Published - Feb 2018

Access to Document

https://doi.org/10.1093/mmy/myx035

https://pure.uva.nl/ws/files/32843910/myx035.pdf

Cite this

Gerritsen, M. G., Brinkman, P., Escobar, N., Bos, L. D., de Heer, K., Meijer, M., Janssen, H.-G., de Cock, H., Wosten, H. A. B., Visser, C. E., van Oers, M. H. J., Sterk, P. J., & Wösten, H. A. B. (2018). Profiling of volatile organic compounds produced by clinical Aspergillus isolates using gas chromatography-mass spectrometry. Medical mycology, 56(2), 253-256. https://doi.org/10.1093/mmy/myx035

@article{b21fd61c3a0e497880a4139359ca008f,

title = "Profiling of volatile organic compounds produced by clinical Aspergillus isolates using gas chromatography-mass spectrometry",

abstract = "Volatile organic compounds (VOCs) in exhaled breath may identify the presence of invasive pulmonary aspergillosis. We aimed to detect VOC profiles emitted by in vitro cultured, clinical Aspergillus isolates using gas chromatography–mass spectrometry (GC-MS). Three clinical Aspergillus isolates and a reference strain were cultured while conidiation was prevented. Headspace samples were analyzed using a standardized method. Breath samples of patients from which the cultures were obtained were checked for the presence of the VOCs found in vitro. Each Aspergillus isolate produced a distinct VOC profile. These profiles could not be confirmed in exhaled breath in vivo.",

author = "M.G. Gerritsen and P. Brinkman and N. Escobar and L.D. Bos and {de Heer}, K. and M. Meijer and H.-G. Janssen and {de Cock}, H. and H.A.B. Wosten and C.E. Visser and {van Oers}, M.H.J. and P.J. Sterk and W{\"o}sten, {H. A. B.}",

year = "2018",

month = feb,

doi = "https://doi.org/10.1093/mmy/myx035",

language = "English",

volume = "56",

pages = "253--256",

journal = "Medical mycology",

issn = "1369-3786",

publisher = "Oxford University Press",

number = "2",

}

Gerritsen, MG, Brinkman, P, Escobar, N, Bos, LD, de Heer, K, Meijer, M, Janssen, H-G, de Cock, H, Wosten, HAB, Visser, CE , van Oers, MHJ , Sterk, PJ & Wösten, HAB 2018, 'Profiling of volatile organic compounds produced by clinical Aspergillus isolates using gas chromatography-mass spectrometry', Medical mycology, vol. 56, no. 2, pp. 253-256. https://doi.org/10.1093/mmy/myx035

TY - JOUR

T1 - Profiling of volatile organic compounds produced by clinical Aspergillus isolates using gas chromatography-mass spectrometry

AU - Gerritsen, M.G.

AU - Brinkman, P.

AU - Escobar, N.

AU - Bos, L.D.

AU - de Heer, K.

AU - Meijer, M.

AU - Janssen, H.-G.

AU - de Cock, H.

AU - Wosten, H.A.B.

AU - Visser, C.E.

AU - van Oers, M.H.J.

AU - Sterk, P.J.

AU - Wösten, H. A. B.

PY - 2018/2

Y1 - 2018/2

N2 - Volatile organic compounds (VOCs) in exhaled breath may identify the presence of invasive pulmonary aspergillosis. We aimed to detect VOC profiles emitted by in vitro cultured, clinical Aspergillus isolates using gas chromatography–mass spectrometry (GC-MS). Three clinical Aspergillus isolates and a reference strain were cultured while conidiation was prevented. Headspace samples were analyzed using a standardized method. Breath samples of patients from which the cultures were obtained were checked for the presence of the VOCs found in vitro. Each Aspergillus isolate produced a distinct VOC profile. These profiles could not be confirmed in exhaled breath in vivo.

AB - Volatile organic compounds (VOCs) in exhaled breath may identify the presence of invasive pulmonary aspergillosis. We aimed to detect VOC profiles emitted by in vitro cultured, clinical Aspergillus isolates using gas chromatography–mass spectrometry (GC-MS). Three clinical Aspergillus isolates and a reference strain were cultured while conidiation was prevented. Headspace samples were analyzed using a standardized method. Breath samples of patients from which the cultures were obtained were checked for the presence of the VOCs found in vitro. Each Aspergillus isolate produced a distinct VOC profile. These profiles could not be confirmed in exhaled breath in vivo.

U2 - https://doi.org/10.1093/mmy/myx035

DO - https://doi.org/10.1093/mmy/myx035

M3 - Article

C2 - 28525576

SN - 1369-3786

VL - 56

SP - 253

EP - 256

JO - Medical mycology

JF - Medical mycology

IS - 2

ER -