Protein kinase D controls actin polymerization and cell motility through phosphorylation of cortactin

Tim Eiseler, Angelika Hausser, Line de Kimpe, Johan van Lint, Klaus Pfizenmaier

Research output: Contribution to journalArticle*Academicpeer-review

Abstract

We here identify protein kinase D (PKD) as an upstream regulator of the F-actin-binding protein cortactin and the Arp actin polymerization machinery. PKD phosphorylates cortactin in vitro and in vivo at serine 298 thereby generating a 14-3-3 binding motif. In vitro, a phosphorylation-deficient cortactin-S298A protein accelerated VCA-Arp-cortactin-mediated synergistic actin polymerization and showed reduced F-actin binding, indicative of enhanced turnover of nucleation complexes. In vivo, cortactin co-localized with the nucleation promoting factor WAVE2, essential for lamellipodia extension, in the actin polymerization zone in Heregulin-treated MCF-7 cells. Using a 3-dye FRET-based approach we further demonstrate that WAVE2-Arp and cortactin prominently interact at these structures. Accordingly, cortactin-S298A significantly enhanced lamellipodia extension and directed cell migration. Our data thus unravel a previously unrecognized mechanism by which PKD controls cancer cell motility
Original languageEnglish
Pages (from-to)18672-18683
JournalJournal of biological chemistry
Volume285
Issue number24
DOIs
Publication statusPublished - 2010

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