Quantitative analysis of myofilament protein phosphorylation in small cardiac biopsies

Ruud Zaremba, Daphne Merkus, Nazha Hamdani, Jos M.J. Lamers, Walter J. Paulus, Cris dos Remedios, Dirk J. Duncker, Ger J.M. Stienen, Jolanda van der Velden

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Phosphorylation of cardiac myofilament proteins represents one of the main post-translational mechanisms that regulate cardiac pump function. Human studies are often limited by the amount of available tissue as biopsies taken during cardiac catheterization weigh only 1 mg (dry weight). Similarly, investigation of time- (or dose-) dependent changes in protein phosphorylation in animal studies is often hampered by tissue availability. The present study describes quantitative analysis of phosphorylation status of multiple myofilament proteins by 2-DE and Pro-Q® Diamond stained gradient gels using minor amounts (-0.5 mg dry weight) of human and pig cardiac tissue.

Original languageEnglish
Pages (from-to)1285-1290
Number of pages6
JournalProteomics - Clinical Applications
Issue number10
Publication statusPublished - 1 Oct 2007


  • 2-DE
  • Cardiac tissue
  • Pro-Q® Diamond stain
  • Protein phosphorylation
  • TCA

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