Relatedness of human and animal Clostridium difficile PCR ribotype 078 isolates determined on the basis of multilocus variable-number tandem-repeat analysis and tetracycline resistance

D. Bakker, J. Corver, C. Harmanus, A. Goorhuis, E. C. Keessen, W. N. Fawley, M. H. Wilcox, E. J. Kuijper

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Totals of 102 and 56 Clostridium difficile type 078 strains of human and porcine origins, respectively, from four European countries were investigated by an optimized multilocus variable-number tandem-repeat analysis (MLVA) and for tetracycline susceptibility. Eighty-five percent of all isolates were genetically related, irrespective of human or porcine origin. Human strains were significantly more resistant to tetracycline than porcine strains. All tetracycline-resistant strains contained the Tn916-like transposon harboring the tet(M) gene. We conclude that strains from human and porcine origins are genetically related, irrespective of the country of origin. This may reflect a lack of diversity and/or common source
Original languageEnglish
Pages (from-to)3744-3749
JournalJournal of clinical microbiology
Issue number10
Publication statusPublished - 2010

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