TY - JOUR
T1 - Reliable measurements of extracellular vesicles by clinical flow cytometry
AU - Kuiper, Martine
AU - van de Nes, Arthur
AU - Nieuwland, Rienk
AU - Varga, Zoltan
AU - van der Pol, Edwin
N1 - Funding Information: This work is part of the project ?METVES II? and received funding from the EMPIR program co-financed by the Participating States and from the European Union's Horizon 2020 research and innovation program. E. van der Pol acknowledges funding from the Netherlands Organisation for Scientific Research-Domain Applied and Engineering Sciences (NWO-TTW), research program VENI 15924. We thank Aleksandra G?secka for providing the data underlying Figure?3. Funding Information: This work is part of the project “METVES II” and received funding from the EMPIR program co‐financed by the Participating States and from the European Union's Horizon 2020 research and innovation program. E. van der Pol acknowledges funding from the Netherlands Organisation for Scientific Research‐Domain Applied and Engineering Sciences (NWO‐TTW), research program VENI 15924. We thank Aleksandra Gąsecka for providing the data underlying Figure 3 . Publisher Copyright: © 2020 The Authors. American Journal of Reproductive Immunology published by John Wiley & Sons Ltd Copyright: Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/2
Y1 - 2021/2
N2 - Extracellular vesicles (EVs) are cell-derived particles with a phospholipid membrane present in all body fluids. Because EV properties change in health and disease, EVs have excellent potential to become biomarkers for diagnosis, prognosis, or monitoring of disease. The only technique capable of detecting, sizing, and phenotyping a million of EVs within minutes is (clinical) flow cytometry. A flow cytometer measures light scattering and fluorescence signals of single EVs. Although these signals contain valuable information about the presence and composition of EVs, the signals are expressed in arbitrary units, which make the comparison of measurement results impossible between instruments and laboratories. Additionally, unintended and undocumented variations in the source, preparation, and analysis of the sample lead to orders of magnitude variations in the measured EV concentrations. Here, we will explain the basics, challenges, and common misconceptions of EV flow cytometry. In addition, we provide an overview of recent standardization initiatives, which are a prerequisite for comparison of clinical data and thus for clinical biomarker exploration of EVs.
AB - Extracellular vesicles (EVs) are cell-derived particles with a phospholipid membrane present in all body fluids. Because EV properties change in health and disease, EVs have excellent potential to become biomarkers for diagnosis, prognosis, or monitoring of disease. The only technique capable of detecting, sizing, and phenotyping a million of EVs within minutes is (clinical) flow cytometry. A flow cytometer measures light scattering and fluorescence signals of single EVs. Although these signals contain valuable information about the presence and composition of EVs, the signals are expressed in arbitrary units, which make the comparison of measurement results impossible between instruments and laboratories. Additionally, unintended and undocumented variations in the source, preparation, and analysis of the sample lead to orders of magnitude variations in the measured EV concentrations. Here, we will explain the basics, challenges, and common misconceptions of EV flow cytometry. In addition, we provide an overview of recent standardization initiatives, which are a prerequisite for comparison of clinical data and thus for clinical biomarker exploration of EVs.
KW - calibration
KW - data interpretation
KW - extracellular vesicles
KW - flow cytometry
KW - standardization
UR - http://www.scopus.com/inward/record.url?scp=85092165892&partnerID=8YFLogxK
U2 - https://doi.org/10.1111/aji.13350
DO - https://doi.org/10.1111/aji.13350
M3 - Article
C2 - 32966654
SN - 1046-7408
VL - 85
SP - e13350
JO - American journal of reproductive immunology (New York, N.Y.
JF - American journal of reproductive immunology (New York, N.Y.
IS - 2
M1 - e13350
ER -