Rhesus macaque and chimpanzee DC-SIGN act as HIV/SIV gp120 trans-receptors, similar to human DC-SIGN

T. B. Geijtenbeek; G. Koopman; G. C. van Duijnhoven; S. J. van Vliet; A. C. van Schijndel; A. Engering; J. L. Heeney; Y. van Kooyk

doi:https://doi.org/10.1016/S0165-2478(01)00279-6

Rhesus macaque and chimpanzee DC-SIGN act as HIV/SIV gp120 trans-receptors, similar to human DC-SIGN

T. B. Geijtenbeek, G. Koopman, G. C. van Duijnhoven, S. J. van Vliet, A. C. van Schijndel, A. Engering, J. L. Heeney, Y. van Kooyk

Research output: Contribution to journal › Article › Academic › peer-review

34 Citations (Scopus)

Abstract

Dendritic cells (DC) have been implicated in the pathogenesis of both human and simian immunodeficiency viruses (HIV and SIV, respectively). The DC-specific HIV-1 trans-receptor DC-SIGN is thought to be essential for viral dissemination by DC. Abundant expression in lymphoid tissues also implies a function for DC-SIGN in chronic HIV-1 infections, in facilitating persistent infection of T cells. We have therefore isolated the rhesus macaque and chimpanzee homologues of DC-SIGN to investigate their function in a primate model. Both rhesus macaque and chimpanzee DC-SIGN are highly similar to the human homologue. Three monoclonal antibodies against human DC-SIGN, AZN-D1, -D2 and -D3, cross-react with rhesus macaque DC-SIGN, whereas AZN-D2 does not cross-react with chimpanzee DC-SIGN. The primate homologues are abundantly expressed in lymphoid tissues such as lymph nodes, as well as in mucosal tissues involved in sexual transmission of HIV-1, and are functionally similar to human DC-SIGN. They have a high affinity for the immunological ligands of DC-SIGN: ICAM-2 and -3. Moreover, both homologues bind the HIV-1 envelope glycoprotein gp120 and therefore can act as a HIV-1 trans-receptor in the same way as human DC-SIGN. These data demonstrate that primate models are suitable to further dissect the role of DC-SIGN in the transmission and pathogenesis of infection with immunodeficiency viruses

Original language	English
Pages (from-to)	101-107
Number of pages	7
Journal	Immunology letters
Volume	79
Issue number	1-2
DOIs	https://doi.org/10.1016/S0165-2478(01)00279-6
Publication status	Published - 1 Nov 2001

Keywords

Amino Acid Sequence
Animals
Antibodies, Monoclonal
Base Sequence
Cell Adhesion Molecules
Cross Reactions
DNA, Complementary/genetics
Dendritic Cells/immunology
Gene Expression
HIV Envelope Protein gp120/immunology
HIV Infections/immunology
HIV-1/immunology
Humans
Lectins, C-Type
Lectins/genetics
Ligands
Macaca mulatta/genetics
Membrane Glycoproteins
Molecular Sequence Data
Pan troglodytes/genetics
Receptors, Cell Surface/genetics
Receptors, HIV/genetics
Sequence Homology, Amino Acid
Simian Immunodeficiency Virus/immunology
Species Specificity
Viral Envelope Proteins

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https://doi.org/10.1016/S0165-2478(01)00279-6

Cite this

@article{be1558a3bb2f41baa43b2a769170d471,

title = "Rhesus macaque and chimpanzee DC-SIGN act as HIV/SIV gp120 trans-receptors, similar to human DC-SIGN",

abstract = "Dendritic cells (DC) have been implicated in the pathogenesis of both human and simian immunodeficiency viruses (HIV and SIV, respectively). The DC-specific HIV-1 trans-receptor DC-SIGN is thought to be essential for viral dissemination by DC. Abundant expression in lymphoid tissues also implies a function for DC-SIGN in chronic HIV-1 infections, in facilitating persistent infection of T cells. We have therefore isolated the rhesus macaque and chimpanzee homologues of DC-SIGN to investigate their function in a primate model. Both rhesus macaque and chimpanzee DC-SIGN are highly similar to the human homologue. Three monoclonal antibodies against human DC-SIGN, AZN-D1, -D2 and -D3, cross-react with rhesus macaque DC-SIGN, whereas AZN-D2 does not cross-react with chimpanzee DC-SIGN. The primate homologues are abundantly expressed in lymphoid tissues such as lymph nodes, as well as in mucosal tissues involved in sexual transmission of HIV-1, and are functionally similar to human DC-SIGN. They have a high affinity for the immunological ligands of DC-SIGN: ICAM-2 and -3. Moreover, both homologues bind the HIV-1 envelope glycoprotein gp120 and therefore can act as a HIV-1 trans-receptor in the same way as human DC-SIGN. These data demonstrate that primate models are suitable to further dissect the role of DC-SIGN in the transmission and pathogenesis of infection with immunodeficiency viruses",

keywords = "Amino Acid Sequence, Animals, Antibodies, Monoclonal, Base Sequence, Cell Adhesion Molecules, Cross Reactions, DNA, Complementary/genetics, Dendritic Cells/immunology, Gene Expression, HIV Envelope Protein gp120/immunology, HIV Infections/immunology, HIV-1/immunology, Humans, Lectins, C-Type, Lectins/genetics, Ligands, Macaca mulatta/genetics, Membrane Glycoproteins, Molecular Sequence Data, Pan troglodytes/genetics, Receptors, Cell Surface/genetics, Receptors, HIV/genetics, Sequence Homology, Amino Acid, Simian Immunodeficiency Virus/immunology, Species Specificity, Viral Envelope Proteins",

author = "Geijtenbeek, {T. B.} and G. Koopman and {van Duijnhoven}, {G. C.} and {van Vliet}, {S. J.} and {van Schijndel}, {A. C.} and A. Engering and Heeney, {J. L.} and {van Kooyk}, Y.",

year = "2001",

month = nov,

day = "1",

doi = "https://doi.org/10.1016/S0165-2478(01)00279-6",

language = "English",

volume = "79",

pages = "101--107",

journal = "Immunology letters",

issn = "0165-2478",

publisher = "Elsevier",

number = "1-2",

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TY - JOUR

T1 - Rhesus macaque and chimpanzee DC-SIGN act as HIV/SIV gp120 trans-receptors, similar to human DC-SIGN

AU - Geijtenbeek, T. B.

AU - Koopman, G.

AU - van Duijnhoven, G. C.

AU - van Vliet, S. J.

AU - van Schijndel, A. C.

AU - Engering, A.

AU - Heeney, J. L.

AU - van Kooyk, Y.

PY - 2001/11/1

Y1 - 2001/11/1

N2 - Dendritic cells (DC) have been implicated in the pathogenesis of both human and simian immunodeficiency viruses (HIV and SIV, respectively). The DC-specific HIV-1 trans-receptor DC-SIGN is thought to be essential for viral dissemination by DC. Abundant expression in lymphoid tissues also implies a function for DC-SIGN in chronic HIV-1 infections, in facilitating persistent infection of T cells. We have therefore isolated the rhesus macaque and chimpanzee homologues of DC-SIGN to investigate their function in a primate model. Both rhesus macaque and chimpanzee DC-SIGN are highly similar to the human homologue. Three monoclonal antibodies against human DC-SIGN, AZN-D1, -D2 and -D3, cross-react with rhesus macaque DC-SIGN, whereas AZN-D2 does not cross-react with chimpanzee DC-SIGN. The primate homologues are abundantly expressed in lymphoid tissues such as lymph nodes, as well as in mucosal tissues involved in sexual transmission of HIV-1, and are functionally similar to human DC-SIGN. They have a high affinity for the immunological ligands of DC-SIGN: ICAM-2 and -3. Moreover, both homologues bind the HIV-1 envelope glycoprotein gp120 and therefore can act as a HIV-1 trans-receptor in the same way as human DC-SIGN. These data demonstrate that primate models are suitable to further dissect the role of DC-SIGN in the transmission and pathogenesis of infection with immunodeficiency viruses

AB - Dendritic cells (DC) have been implicated in the pathogenesis of both human and simian immunodeficiency viruses (HIV and SIV, respectively). The DC-specific HIV-1 trans-receptor DC-SIGN is thought to be essential for viral dissemination by DC. Abundant expression in lymphoid tissues also implies a function for DC-SIGN in chronic HIV-1 infections, in facilitating persistent infection of T cells. We have therefore isolated the rhesus macaque and chimpanzee homologues of DC-SIGN to investigate their function in a primate model. Both rhesus macaque and chimpanzee DC-SIGN are highly similar to the human homologue. Three monoclonal antibodies against human DC-SIGN, AZN-D1, -D2 and -D3, cross-react with rhesus macaque DC-SIGN, whereas AZN-D2 does not cross-react with chimpanzee DC-SIGN. The primate homologues are abundantly expressed in lymphoid tissues such as lymph nodes, as well as in mucosal tissues involved in sexual transmission of HIV-1, and are functionally similar to human DC-SIGN. They have a high affinity for the immunological ligands of DC-SIGN: ICAM-2 and -3. Moreover, both homologues bind the HIV-1 envelope glycoprotein gp120 and therefore can act as a HIV-1 trans-receptor in the same way as human DC-SIGN. These data demonstrate that primate models are suitable to further dissect the role of DC-SIGN in the transmission and pathogenesis of infection with immunodeficiency viruses

KW - Amino Acid Sequence

KW - Animals

KW - Antibodies, Monoclonal

KW - Base Sequence

KW - Cell Adhesion Molecules

KW - Cross Reactions

KW - DNA, Complementary/genetics

KW - Dendritic Cells/immunology

KW - Gene Expression

KW - HIV Envelope Protein gp120/immunology

KW - HIV Infections/immunology

KW - HIV-1/immunology

KW - Humans

KW - Lectins, C-Type

KW - Lectins/genetics

KW - Ligands

KW - Macaca mulatta/genetics

KW - Membrane Glycoproteins

KW - Molecular Sequence Data

KW - Pan troglodytes/genetics

KW - Receptors, Cell Surface/genetics

KW - Receptors, HIV/genetics

KW - Sequence Homology, Amino Acid

KW - Simian Immunodeficiency Virus/immunology

KW - Species Specificity

KW - Viral Envelope Proteins

U2 - https://doi.org/10.1016/S0165-2478(01)00279-6

DO - https://doi.org/10.1016/S0165-2478(01)00279-6

M3 - Article

C2 - 11595296

SN - 0165-2478

VL - 79

SP - 101

EP - 107

JO - Immunology letters

JF - Immunology letters

IS - 1-2

ER -