Background: The aim of this study is to evaluate feasibility and safety of robotic-assisted donor nephrectomy (RADN) as compared with the hand-assisted laparoscopic technique (HALDN). Patient and methods: This is a retrospective study of the first 83 consecutive RADN procedures which were compared with a group of 103 consecutive HALDN procedures. Results: The median operating time in the RADN group and the HALDN group were respectively 173 (SD = 37) and 137 (SD = 29) min (p = 0.000). The mean first warm ischemia time (min, SD) in the RADN and HALDN group were respectively 2.60 (0.78) and 2.15 (0.38) (p = 0.000). The median blood loss (ml, SD) in the RADN and HALDN group were respectively 33 (87) and 64 (146) ml (p = 0.000). There were no statistically significant differences in post-operative pain-score, post-operative length of stay and complications between the two groups. There were also no statistically significant differences in recipient serum creatinine at 1 week and 1 month after transplantation. The median recipients eGFR (CKD-EPI) at 1 week in the RADN and HALDN group were respectively 50 (SD = 20) and 38 (SD = 19) (p = 0.018) and at 1 month respectively 52 (SD = 16) and 47 (SD = 15) ml/min/1.73m2 (p = 0.090). Conclusion: Robotic-assisted donor nephrectomy is an evolving technique which is safe for the donor and for the graft.