TY - JOUR
T1 - Semi-empirical model of the effect of scattering on single fiber fluorescence intensity measured on a turbid medium
AU - Kanick, S. C.
AU - Robinson, D. J.
AU - Sterenborg, H. J. C. M.
AU - Amelink, A.
PY - 2012
Y1 - 2012
N2 - Quantitative determination of fluorophore content from fluorescence measurements in turbid media, such as tissue, is complicated by the influence of scattering properties on the collected signal. This study utilizes a Monte Carlo model to characterize the relationship between the fluorescence intensity collected by a single fiber optic probe (F-SF) and the scattering properties. Simulations investigate a wide range of biologically relevant scattering properties specified independently at excitation (lambda(x)) and emission (lambda(m)) wavelengths, including reduced scattering coefficients in the range mu(s)'(lambda(x)) is an element of [0.1-8]mm(-1) and mu(s)'(lambda(m)) is an element of [0.25-1] x mu(s)'(lambda(x)). Investigated scattering phase functions (P(theta)) include both Henyey-Greenstein and Modified Henyey-Greenstein forms, and a wide range of fiber diameters (d(f) is an element of [0.2-1.0] mm) was simulated. A semi-empirical model is developed to estimate the collected F-SF as the product of an effective sampling volume, and the effective excitation fluence and the effective escape probability within the effective sampling volume. The model accurately estimates F-SF intensities (r=0.999) over the investigated range of mu(s)'(lambda(x)) and mu(s)'(lambda(m)), is insensitive to the form of the P(theta), and provides novel insight into a dimensionless relationship linking F-SF measured by different d(f). (C) 2011 Optical Society of America
AB - Quantitative determination of fluorophore content from fluorescence measurements in turbid media, such as tissue, is complicated by the influence of scattering properties on the collected signal. This study utilizes a Monte Carlo model to characterize the relationship between the fluorescence intensity collected by a single fiber optic probe (F-SF) and the scattering properties. Simulations investigate a wide range of biologically relevant scattering properties specified independently at excitation (lambda(x)) and emission (lambda(m)) wavelengths, including reduced scattering coefficients in the range mu(s)'(lambda(x)) is an element of [0.1-8]mm(-1) and mu(s)'(lambda(m)) is an element of [0.25-1] x mu(s)'(lambda(x)). Investigated scattering phase functions (P(theta)) include both Henyey-Greenstein and Modified Henyey-Greenstein forms, and a wide range of fiber diameters (d(f) is an element of [0.2-1.0] mm) was simulated. A semi-empirical model is developed to estimate the collected F-SF as the product of an effective sampling volume, and the effective excitation fluence and the effective escape probability within the effective sampling volume. The model accurately estimates F-SF intensities (r=0.999) over the investigated range of mu(s)'(lambda(x)) and mu(s)'(lambda(m)), is insensitive to the form of the P(theta), and provides novel insight into a dimensionless relationship linking F-SF measured by different d(f). (C) 2011 Optical Society of America
U2 - https://doi.org/10.1364/BOE.3.000137
DO - https://doi.org/10.1364/BOE.3.000137
M3 - Article
C2 - 22254174
SN - 2156-7085
VL - 3
SP - 137
EP - 152
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 1
ER -