TY - JOUR
T1 - Sequence-independent VIDISCA-454 technique to discover new viruses in canine livers
AU - van der Heijden, Mitzi
AU - de Vries, Michel
AU - van Steenbeek, Frank G.
AU - Favier, Robert P.
AU - Deijs, Martin
AU - Brinkhof, Bas
AU - Rothuizen, Jan
AU - van der Hoek, Lia
AU - Penning, Louis C.
PY - 2012
Y1 - 2012
N2 - In many mammals, viruses cause hepatitis. Despite many efforts a specific virus responsible for canine idiopathic hepatitis has not been identified. The discovery of a viral etiology in canine hepatitis will promote the development of specific drugs and vaccines for the treatment of idiopathic hepatitis in dogs. The objective of this study was the application of the sequence-independent Virus Discovery cDNA-amplified fragment length polymorphism (VIDISCA) technique combined with high through-put sequencing on a Roche-454 sequencer to identify unknown viruses. Liver tissue of a dog with idiopathic acute hepatitis was cultured on a canine liver cell line and the cell culture medium was submitted to the VIDISCA-454 technique. Without prior knowledge of the viral species involved, this technique identified Canine adenoyirus type 1 (CAV-1) as the infecting agent. This demonstrates the power of VIDISCA-454 to identify viruses, independent of preliminary information about the genomic sequence. Consequently, the strategy of propagation in this cell line followed by the VIDISCA-454 technique is valuable to identify the viral etiology of idiopathic hepatitis in dogs. (C) 2012 Elsevier B.V. All rights reserved
AB - In many mammals, viruses cause hepatitis. Despite many efforts a specific virus responsible for canine idiopathic hepatitis has not been identified. The discovery of a viral etiology in canine hepatitis will promote the development of specific drugs and vaccines for the treatment of idiopathic hepatitis in dogs. The objective of this study was the application of the sequence-independent Virus Discovery cDNA-amplified fragment length polymorphism (VIDISCA) technique combined with high through-put sequencing on a Roche-454 sequencer to identify unknown viruses. Liver tissue of a dog with idiopathic acute hepatitis was cultured on a canine liver cell line and the cell culture medium was submitted to the VIDISCA-454 technique. Without prior knowledge of the viral species involved, this technique identified Canine adenoyirus type 1 (CAV-1) as the infecting agent. This demonstrates the power of VIDISCA-454 to identify viruses, independent of preliminary information about the genomic sequence. Consequently, the strategy of propagation in this cell line followed by the VIDISCA-454 technique is valuable to identify the viral etiology of idiopathic hepatitis in dogs. (C) 2012 Elsevier B.V. All rights reserved
U2 - https://doi.org/10.1016/j.jviromet.2012.05.019
DO - https://doi.org/10.1016/j.jviromet.2012.05.019
M3 - Article
C2 - 22664180
SN - 0166-0934
VL - 185
SP - 152
EP - 155
JO - Journal of virological methods
JF - Journal of virological methods
IS - 1
ER -