TY - JOUR
T1 - Soluble granzymes are released during human endotoxemia and in patients with severe infection due to gram-negative bacteria
AU - Lauw, F.N.
AU - Simpson, A.J.H.
AU - Hack, C.E.
AU - Prins, J.M.
AU - Wolbink, A.M.
AU - van Deventer, S.J.H.
AU - Chaowagul, W.
AU - White, N.J.
AU - van der Poll, T.
N1 - inf/12
PY - 2000
Y1 - 2000
N2 - Extracellular release of granzymes is considered to reflect the involvement of cytotoxic T lymphocytes and NK cells in various disease states. To obtain insight into granzyme release during bacterial infection, granzyme levels were measured during experimental human endotoxemia and in patients with melioidosis, a severe infection due to gram-negative bacteria. Plasma concentrations of granzyme A (GrA) and GrB increased transiently after endotoxin administration, peaking after 2-6 h. In patients with bacteremic melioidosis, GrA and GrB levels were elevated on admission and remained high during the 72-h study period. In whole blood stimulated with heat-killed Burkholderia pseudomallei, neutralization of tumor necrosis factor, interleukin-12, or interleukin-18 inhibited granzyme secretion, which was independent of interferon-gamma. Stimulation with endotoxin and other gram-negative and gram-positive bacteria also strongly induced the secretion of granzymes, suggesting that granzyme release is a general immune response during bacterial infection. The interaction between the cytokine network and granzymes may play an important immunoregulatory role during bacterial infections
AB - Extracellular release of granzymes is considered to reflect the involvement of cytotoxic T lymphocytes and NK cells in various disease states. To obtain insight into granzyme release during bacterial infection, granzyme levels were measured during experimental human endotoxemia and in patients with melioidosis, a severe infection due to gram-negative bacteria. Plasma concentrations of granzyme A (GrA) and GrB increased transiently after endotoxin administration, peaking after 2-6 h. In patients with bacteremic melioidosis, GrA and GrB levels were elevated on admission and remained high during the 72-h study period. In whole blood stimulated with heat-killed Burkholderia pseudomallei, neutralization of tumor necrosis factor, interleukin-12, or interleukin-18 inhibited granzyme secretion, which was independent of interferon-gamma. Stimulation with endotoxin and other gram-negative and gram-positive bacteria also strongly induced the secretion of granzymes, suggesting that granzyme release is a general immune response during bacterial infection. The interaction between the cytokine network and granzymes may play an important immunoregulatory role during bacterial infections
KW - AMC wi-co
U2 - https://doi.org/10.1086/315642
DO - https://doi.org/10.1086/315642
M3 - Article
C2 - 10882599
SN - 0022-1899
VL - 182
SP - 206
EP - 213
JO - The Journal of Infectious Diseases
JF - The Journal of Infectious Diseases
IS - 1
ER -