Solutions for hematocrit bias in dried blood spot hormone analysis

Vera de Kleijne; Isabelle Kohler; Annemieke C. Heijboer; Mariëtte T. Ackermans

doi:https://doi.org/10.4155/bio-2021-0119

Solutions for hematocrit bias in dried blood spot hormone analysis

Vera de Kleijne, Isabelle Kohler, Annemieke C. Heijboer, Mariëtte T. Ackermans

Research output: Contribution to journal › Review article › Academic › peer-review

6 Citations (Scopus)

Abstract

Over the last years, dried blood spot (DBS) sampling has gained significant interest due to development of analytical techniques combined with DBS, the simplicity and low cost of the method. Despite its wide use, DBS sampling can lead to inaccurate results due to the impact of the hematocrit (Hct) on the analysis. Some analytes have shown to be hardly impacted by Hct values. However, in other cases, a significant impact of Hct is observed, which requires the use of alternative approaches to circumvent this issue. This review describes the possible impact of Hct-related bias in DBS sampling in the context of hormone analysis and discusses the different methodologies that can be used to overcome this bias to ensure accurate results.

Original language	English
Pages (from-to)	1293-1308
Number of pages	16
Journal	BIOANALYSIS
Volume	13
Issue number	16
DOIs	https://doi.org/10.4155/bio-2021-0119
Publication status	Published - 1 Aug 2021

Keywords

dried blood spot
hematocrit
hormone analysis

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https://doi.org/10.4155/bio-2021-0119

Cite this

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title = "Solutions for hematocrit bias in dried blood spot hormone analysis",

abstract = "Over the last years, dried blood spot (DBS) sampling has gained significant interest due to development of analytical techniques combined with DBS, the simplicity and low cost of the method. Despite its wide use, DBS sampling can lead to inaccurate results due to the impact of the hematocrit (Hct) on the analysis. Some analytes have shown to be hardly impacted by Hct values. However, in other cases, a significant impact of Hct is observed, which requires the use of alternative approaches to circumvent this issue. This review describes the possible impact of Hct-related bias in DBS sampling in the context of hormone analysis and discusses the different methodologies that can be used to overcome this bias to ensure accurate results.",

keywords = "dried blood spot, hematocrit, hormone analysis",

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T1 - Solutions for hematocrit bias in dried blood spot hormone analysis

AU - de Kleijne, Vera

AU - Kohler, Isabelle

AU - Heijboer, Annemieke C.

AU - Ackermans, Mariëtte T.

PY - 2021/8/1

Y1 - 2021/8/1

N2 - Over the last years, dried blood spot (DBS) sampling has gained significant interest due to development of analytical techniques combined with DBS, the simplicity and low cost of the method. Despite its wide use, DBS sampling can lead to inaccurate results due to the impact of the hematocrit (Hct) on the analysis. Some analytes have shown to be hardly impacted by Hct values. However, in other cases, a significant impact of Hct is observed, which requires the use of alternative approaches to circumvent this issue. This review describes the possible impact of Hct-related bias in DBS sampling in the context of hormone analysis and discusses the different methodologies that can be used to overcome this bias to ensure accurate results.

AB - Over the last years, dried blood spot (DBS) sampling has gained significant interest due to development of analytical techniques combined with DBS, the simplicity and low cost of the method. Despite its wide use, DBS sampling can lead to inaccurate results due to the impact of the hematocrit (Hct) on the analysis. Some analytes have shown to be hardly impacted by Hct values. However, in other cases, a significant impact of Hct is observed, which requires the use of alternative approaches to circumvent this issue. This review describes the possible impact of Hct-related bias in DBS sampling in the context of hormone analysis and discusses the different methodologies that can be used to overcome this bias to ensure accurate results.

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KW - hematocrit

KW - hormone analysis

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Solutions for hematocrit bias in dried blood spot hormone analysis

Abstract

Keywords

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