TY - JOUR
T1 - Specific expression of GPR56 by human cytotoxic lymphocytes
AU - Peng, Yen-Ming
AU - van de Garde, Martijn D. B.
AU - Cheng, Kai-Fong
AU - Baars, Paul A.
AU - Remmerswaal, Ester B. M.
AU - van Lier, René A. W.
AU - Mackay, Charles R.
AU - Lin, Hsi-Hsien
AU - Hamann, Jörg
PY - 2011
Y1 - 2011
N2 - We here report the existence of a new cluster of adhesion-GPCRs in human immune cells. Analysis of a comprehensive immune cell transcriptome dataset indicated that expression of the closely related receptors, GPR56, GPR97, and GPR114, is associated with single lymphocyte and granulocyte subsets. Applying flow cytometric analysis with newly generated mAb, we show that expression of GPR56 is restricted to cytotoxic NK and T lymphocytes, including CD8(+), CD4(+), and γδ T cells. Primary infection with human CMV, which generates a vast population of CD8(+) T cells with an effector phenotype, induced a strong increase in GPR56 expression in virus-specific CD8(+) T cells that remained detectable during latency. In NK-92 cells, ectopic expression of GPR56 inhibited spontaneous and SDF-1-stimulated cell migration. Our data suggest that GPR56 expression is a common trait of human cytotoxic lymphocytes and might affect the migratory properties of these cells
AB - We here report the existence of a new cluster of adhesion-GPCRs in human immune cells. Analysis of a comprehensive immune cell transcriptome dataset indicated that expression of the closely related receptors, GPR56, GPR97, and GPR114, is associated with single lymphocyte and granulocyte subsets. Applying flow cytometric analysis with newly generated mAb, we show that expression of GPR56 is restricted to cytotoxic NK and T lymphocytes, including CD8(+), CD4(+), and γδ T cells. Primary infection with human CMV, which generates a vast population of CD8(+) T cells with an effector phenotype, induced a strong increase in GPR56 expression in virus-specific CD8(+) T cells that remained detectable during latency. In NK-92 cells, ectopic expression of GPR56 inhibited spontaneous and SDF-1-stimulated cell migration. Our data suggest that GPR56 expression is a common trait of human cytotoxic lymphocytes and might affect the migratory properties of these cells
U2 - https://doi.org/10.1189/jlb.0211092
DO - https://doi.org/10.1189/jlb.0211092
M3 - Article
C2 - 21724806
SN - 0741-5400
VL - 90
SP - 735
EP - 740
JO - Journal of leukocyte biology
JF - Journal of leukocyte biology
IS - 4
ER -