Stimulation of D-2 dopamine receptors decreases the evoked in vitro release of [3H]acetylcholine from rat neostriatum: role of K+ and Ca2+

B Drukarch, E Schepens, A N Schoffelmeer, J C Stoof

Research output: Contribution to journalArticleAcademicpeer-review

53 Citations (Scopus)


Reportedly, stimulation of D-2 dopamine receptors inhibits the depolarization-induced release of acetylcholine from the neostriatum in a cyclic AMP-independent manner. In the present study, we investigated the role of K+ and Ca2+ in the D-2 receptor-mediated inhibition of evoked [3H]acetylcholine release from rat striatal tissue slices. It is shown that the D-2 receptor-mediated decrease of K+-evoked [3H]acetylcholine release is not influenced by the extracellular Ca2+ concentration. However, increasing extracellular K+, in the presence and absence of Ca2+, markedly attenuates the effect of D-2 stimulation on the K+-evoked [3H]acetylcholine release. Furthermore, it is shown that activation of D-2 receptors in the absence of Ca2+ also inhibits the veratrine-evoked release of [3H]acetylcholine from rat striatum. These results suggest that the D-2 dopamine receptor mediates the decrease of depolarization-induced [3H]acetylcholine release from rat striatum primarily by stimulation of K+ efflux (opening of K+ channels) and inhibition of intracellular Ca2+ mobilization.

Original languageEnglish
Pages (from-to)1680-5
Number of pages6
JournalJournal of neurochemistry
Issue number6
Publication statusPublished - Jun 1989


  • Acetylcholine
  • Animals
  • Calcium
  • Corpus Striatum
  • Dopamine Agents
  • Ergolines
  • In Vitro Techniques
  • Journal Article
  • Male
  • Potassium
  • Quinpirole
  • Rats
  • Rats, Inbred Strains
  • Receptors, Dopamine
  • Receptors, Dopamine D2
  • Stimulation, Chemical
  • Tritium
  • Veratrine

Cite this