TY - JOUR
T1 - Streptococcus suis outbreak caused by an emerging zoonotic strain with acquired multi-drug resistance in Thailand
AU - Brizuela, Jaime
AU - Kajeekul, Rattagan
AU - Roodsant, Thomas J.
AU - Riwload, Athita
AU - Boueroy, Parichart
AU - Pattanapongpaibool, Auttapong
AU - Thaipadungpanit, Janjira
AU - Jenjaroenpun, Piroon
AU - Wongsurawat, Thidathip
AU - Batty, Elizabeth M.
AU - van der Putten, Boas C. L.
AU - Schultsz, Constance
AU - Kerdsin, Anusak
N1 - Funding Information: This work was supported by the CANVAS research project (grant number: LSHM19137), a public private partnership powered by Health~Holland, Top Sector Life Sciences & Health, a research and innovation funding program of the Dutch government. Authors, JB, TR and CS are associated with the CANVAS research project. Publisher Copyright: © 2023 The Authors.
PY - 2023/2/15
Y1 - 2023/2/15
N2 - Streptococcus suis is an emerging zoonotic swine pathogen which can cause severe infections in humans. In March 2021, an outbreak of S. suis infections with 19 confirmed cases of septicemia and meningitis leading to two deaths, occurred in Nakhon Ratchasima province, Thailand. We characterized the outbreak through an epidemiological investigation combined with Illumina and Nanopore whole genome sequencing (WGS). The source of the outbreak was traced back to a raw pork dish prepared from a single pig during a Buddhist ceremony attended by 241 people. WGS analysis revealed that a single S. suis serotype 2 strain belonging to a novel sequence type (ST) of the emergent Thai zoonotic clade CC233/379, was responsible for the infections. The outbreak clone grouped together with other Thai zoonotic strains from CC233/379 and CC104 in a global S. suis phylogeny and capsule switching events between serotype 2 zoonotic strains and serotype 7 porcine strains were identified. The outbreak strain showed reduced susceptibility to penicillin corresponding with mutations in key residues in the penicillin binding proteins (PBPs). Furthermore, the outbreak strain was resistant to tetracycline, erythromycin, clindamycin, linezolid and chloramphenicol, having acquired an integrative and conjugative element (ICE) carrying resistance genes tetO and ermB, as well as a transposon from the IS1216 family carrying optrA and ermA. This investigation demonstrates that multi-drug resistant zoonotic lineages of S. suis which pose a threat to human health continue to emerge.
AB - Streptococcus suis is an emerging zoonotic swine pathogen which can cause severe infections in humans. In March 2021, an outbreak of S. suis infections with 19 confirmed cases of septicemia and meningitis leading to two deaths, occurred in Nakhon Ratchasima province, Thailand. We characterized the outbreak through an epidemiological investigation combined with Illumina and Nanopore whole genome sequencing (WGS). The source of the outbreak was traced back to a raw pork dish prepared from a single pig during a Buddhist ceremony attended by 241 people. WGS analysis revealed that a single S. suis serotype 2 strain belonging to a novel sequence type (ST) of the emergent Thai zoonotic clade CC233/379, was responsible for the infections. The outbreak clone grouped together with other Thai zoonotic strains from CC233/379 and CC104 in a global S. suis phylogeny and capsule switching events between serotype 2 zoonotic strains and serotype 7 porcine strains were identified. The outbreak strain showed reduced susceptibility to penicillin corresponding with mutations in key residues in the penicillin binding proteins (PBPs). Furthermore, the outbreak strain was resistant to tetracycline, erythromycin, clindamycin, linezolid and chloramphenicol, having acquired an integrative and conjugative element (ICE) carrying resistance genes tetO and ermB, as well as a transposon from the IS1216 family carrying optrA and ermA. This investigation demonstrates that multi-drug resistant zoonotic lineages of S. suis which pose a threat to human health continue to emerge.
KW - Streptococcus suis
KW - antimicrobial resistance
KW - outbreak
KW - whole genome sequencing
KW - zoonosis
UR - http://www.scopus.com/inward/record.url?scp=85148250439&partnerID=8YFLogxK
U2 - https://doi.org/10.1099/mgen.0.000952
DO - https://doi.org/10.1099/mgen.0.000952
M3 - Article
C2 - 36790403
SN - 2057-5858
VL - 9
JO - Microbial genomics
JF - Microbial genomics
IS - 2
M1 - 000952
ER -