TY - JOUR
T1 - Sublingual microcirculation
T2 - comparison between the 415 nm blue light and 520 nm green light of sidestream dark field videomicroscopes
AU - Liu, Bo
AU - He, Huaiwu
AU - Feng, Xiaokai
AU - Yuan, Siyi
AU - Long, Yun
AU - Akin, Şakir
AU - Ince, Can
N1 - Funding Information: This work was supported by Peking Union Medical College Hospital of Medical Novel Medical Technology Project (No. XJS20190210), Excellence Program of Key Clinical Specialty of Critical Care Medicine in Beijing. Publisher Copyright: © 2022, The Author(s), under exclusive licence to Springer Nature B.V.
PY - 2022
Y1 - 2022
N2 - Green light with a wavelength of 520 nm is commonly used in sidestream dark field (SDF) video microscopes for sublingual microcirculation assessment in clinical practice. However, blue light could obtain a clearer microcirculatory image due to a higher light absorption coefficient of hemoglobin. The aim of this study was to compare the sublingual microcirculatory image quality acquisition and related microcirculatory parameters between 520 nm green light and 415 nm blue light probes in the SDF device named MicroSee V100. Sublingual microcirculation films from twenty-one healthy volunteers were prospectively collected by blue light and green light probes, and only one video of each wavelength was recorded and analyzed in each volunteer. Moreover, 200 sublingual microcirculation films (100 by blue light probe and 100 by green light probe) of ICU patients were retrospectively scored for microcirculation image quality. Compared to green light, an increase in the perfused vessel density (paired t test, increased by 4.6 ± 4.7 mm/mm2, P < 0.0001) and total vessel density (paired t test, increased by 5.1 ± 4.6 mm/mm2, P < 0.0001) was observed by blue light in the healthy volunteers. The blue light probe had a significantly lower rate of unacceptable films than the green light probe in the 200 films of ICU patients (10/100 vs. 39/100, P < 0.0001). Blue light provides a higher microcirculatory vessel density and image quality than the existing SDF probe using green light.
AB - Green light with a wavelength of 520 nm is commonly used in sidestream dark field (SDF) video microscopes for sublingual microcirculation assessment in clinical practice. However, blue light could obtain a clearer microcirculatory image due to a higher light absorption coefficient of hemoglobin. The aim of this study was to compare the sublingual microcirculatory image quality acquisition and related microcirculatory parameters between 520 nm green light and 415 nm blue light probes in the SDF device named MicroSee V100. Sublingual microcirculation films from twenty-one healthy volunteers were prospectively collected by blue light and green light probes, and only one video of each wavelength was recorded and analyzed in each volunteer. Moreover, 200 sublingual microcirculation films (100 by blue light probe and 100 by green light probe) of ICU patients were retrospectively scored for microcirculation image quality. Compared to green light, an increase in the perfused vessel density (paired t test, increased by 4.6 ± 4.7 mm/mm2, P < 0.0001) and total vessel density (paired t test, increased by 5.1 ± 4.6 mm/mm2, P < 0.0001) was observed by blue light in the healthy volunteers. The blue light probe had a significantly lower rate of unacceptable films than the green light probe in the 200 films of ICU patients (10/100 vs. 39/100, P < 0.0001). Blue light provides a higher microcirculatory vessel density and image quality than the existing SDF probe using green light.
KW - Microvascular flow index
KW - Perfused vessel density
KW - Proportion of perfused vessels
KW - Sidestream dark field
KW - Sublingual microcirculation
KW - Total vessel density
UR - http://www.scopus.com/inward/record.url?scp=85134327752&partnerID=8YFLogxK
U2 - https://doi.org/10.1007/s10877-022-00891-5
DO - https://doi.org/10.1007/s10877-022-00891-5
M3 - Article
C2 - 35838871
SN - 1387-1307
JO - Journal of clinical monitoring and computing
JF - Journal of clinical monitoring and computing
ER -