Super-Resolution Imaging of Intracellular Lipid Nanocarriers to Study Drug Delivery in Photodynamic Therapy

Enzo M. Scutigliani, Jakub A. Kochan, Emilie C. B. Desclos, Art Jonker, Michal Heger, Przemek M. Krawczyk

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review


Liposomal nanocarriers are intensively investigated as delivery vehicles for photoactivatable agents used in photodynamic therapy (PDT). The uptake, intracellular distribution, and processing of the nanocarriers are of paramount importance for the effectiveness of the therapy; visualization and analysis of these processes can, therefore, stimulate the development of improved PDT modalities. Here we describe a simple protocol, based on super-resolution imaging, that can be used for detailed quantification of concentration, distribution, and size of individual lipid nanocarriers in adherent mammalian cells.
Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages7
Publication statusPublished - 2022

Publication series

NameMethods in Molecular Biology


  • Photodynamic therapy
  • Photosensitizers
  • Super-resolution microscopy

Cite this