TY - CHAP
T1 - Super-Resolution Imaging of Intracellular Lipid Nanocarriers to Study Drug Delivery in Photodynamic Therapy
AU - Scutigliani, Enzo M.
AU - Kochan, Jakub A.
AU - Desclos, Emilie C. B.
AU - Jonker, Art
AU - Heger, Michal
AU - Krawczyk, Przemek M.
N1 - Publisher Copyright: © 2022, Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2022
Y1 - 2022
N2 - Liposomal nanocarriers are intensively investigated as delivery vehicles for photoactivatable agents used in photodynamic therapy (PDT). The uptake, intracellular distribution, and processing of the nanocarriers are of paramount importance for the effectiveness of the therapy; visualization and analysis of these processes can, therefore, stimulate the development of improved PDT modalities. Here we describe a simple protocol, based on super-resolution imaging, that can be used for detailed quantification of concentration, distribution, and size of individual lipid nanocarriers in adherent mammalian cells.
AB - Liposomal nanocarriers are intensively investigated as delivery vehicles for photoactivatable agents used in photodynamic therapy (PDT). The uptake, intracellular distribution, and processing of the nanocarriers are of paramount importance for the effectiveness of the therapy; visualization and analysis of these processes can, therefore, stimulate the development of improved PDT modalities. Here we describe a simple protocol, based on super-resolution imaging, that can be used for detailed quantification of concentration, distribution, and size of individual lipid nanocarriers in adherent mammalian cells.
KW - Photodynamic therapy
KW - Photosensitizers
KW - Super-resolution microscopy
UR - http://www.scopus.com/inward/record.url?scp=85129629407&partnerID=8YFLogxK
U2 - https://doi.org/10.1007/978-1-0716-2099-1_37
DO - https://doi.org/10.1007/978-1-0716-2099-1_37
M3 - Chapter
C2 - 35505042
VL - 2451
T3 - Methods in Molecular Biology
SP - 703
EP - 709
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -