TY - JOUR
T1 - Synchronized Rayleigh and Raman scattering for the characterization of single optically trapped extracellular vesicles
AU - Enciso-Martinez, Agustin
AU - van der Pol, Edwin
AU - Lenferink, Aufried T. M.
AU - Terstappen, Leon W. M. M.
AU - van Leeuwen, Ton G.
AU - Otto, Cees
PY - 2020/2
Y1 - 2020/2
N2 - Extracellular Vesicles (EVs) can be used as biomarkers in diseases like cancer, as their lineage of origin and molecular composition depend on the presence of cancer cells. Recognition of tumor-derived EVs (tdEVs) from other particles and EVs in body fluids requires characterization of single EVs to exploit their biomarker potential. We present here a new method based on synchronized Rayleigh and Raman light scattering from a single laser beam, which optically traps single EVs. Rapidly measured sequences of the Rayleigh scattering amplitude show precisely when an individual EV is trapped and the synchronously acquired Raman spectrum labels every time interval with chemical information. Raman spectra of many single EVs can thus be acquired with great fidelity in an automated manner by blocking the laser beam at regular time intervals. This new method enables single EV characterization from fluids at the single particle level.
AB - Extracellular Vesicles (EVs) can be used as biomarkers in diseases like cancer, as their lineage of origin and molecular composition depend on the presence of cancer cells. Recognition of tumor-derived EVs (tdEVs) from other particles and EVs in body fluids requires characterization of single EVs to exploit their biomarker potential. We present here a new method based on synchronized Rayleigh and Raman light scattering from a single laser beam, which optically traps single EVs. Rapidly measured sequences of the Rayleigh scattering amplitude show precisely when an individual EV is trapped and the synchronously acquired Raman spectrum labels every time interval with chemical information. Raman spectra of many single EVs can thus be acquired with great fidelity in an automated manner by blocking the laser beam at regular time intervals. This new method enables single EV characterization from fluids at the single particle level.
KW - Exosomes
KW - Extracellular vesicles
KW - Nanomedicine
KW - Nanoparticles
KW - Raman spectroscopy
KW - Single particle analysis
UR - http://www.scopus.com/inward/record.url?scp=85074592362&partnerID=8YFLogxK
U2 - https://doi.org/10.1016/j.nano.2019.102109
DO - https://doi.org/10.1016/j.nano.2019.102109
M3 - Article
C2 - 31669420
SN - 1549-9634
VL - 24
JO - Nanomedicine: Nanotechnology, Biology and Medicine
JF - Nanomedicine: Nanotechnology, Biology and Medicine
M1 - 102109
ER -