Systemic anti-IFN-gamma treatment and role of macrophage subsets in the foreign body reaction to dermal sheep collagen in rats

I M Khouw, P B van Wachem, R J van der Worp, T K van den Berg, L F de Leij, M J van Luyn

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The application of a biomaterial induces a foreign body reaction. By controlling this reaction, biocompatibility could be improved. We previously demonstrated that impregnation of a biodegradable biomaterial with antibodies against interferon-gamma (IFN-gamma) inhibits the foreign body reaction. In this study we investigate whether systemic administration of the antibody can induce similar reactions. Several parameters are compared between control and anti-IFN-gamma-treated rats: cellular ingrowth; degradation of the biomaterial; ingrowth of macrophage (MO) subsets, T cells, B cells, NK cells, and granulocytes; and expression of the major histocompatibility complex class II (MHC class II) molecule on antigen presenting cells. Treatment with anti-IFN-gamma results in increased cellular ingrowth and biomaterial degradation and a decreased expression of MHC class II. Overall, systemic treatment with anti-IFN-gamma is insufficient to modulate the foreign body reaction. This suggests an alternative mechanism for MO activation besides IFN-gamma. The role of T cells and MO subsets in the foreign body reaction is discussed.

Original languageEnglish
Pages (from-to)297-304
Number of pages8
JournalBiomedical materials (Bristol, England)
Issue number3
Publication statusPublished - 5 Mar 2000


  • Animals
  • Antibodies/administration & dosage
  • Biocompatible Materials/toxicity
  • Collagen/immunology
  • Foreign-Body Reaction/immunology
  • Interferon-gamma/antagonists & inhibitors
  • Macrophage Activation
  • Macrophages/classification
  • Male
  • Rats
  • Sheep
  • Skin/immunology

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