TY - JOUR
T1 - Tetracyclines Disturb Mitochondrial Function across Eukaryotic Models: A Call for Caution in Biomedical Research
AU - Moullan, Norman
AU - Mouchiroud, Laurent
AU - Wang, Xu
AU - Ryu, Dongryeol
AU - Williams, Evan G.
AU - Mottis, Adrienne
AU - Jovaisaite, Virginija
AU - Frochaux, Michael V.
AU - Quiros, Pedro M.
AU - Deplancke, Bart
AU - Houtkooper, Riekelt H.
AU - Auwerx, Johan
PY - 2015
Y1 - 2015
N2 - In recent years, tetracyclines, such as doxycycline, have become broadly used to control gene expression by virtue of the Tet-on/Tet-off systems. However, the wide range of direct effects of tetracycline use has not been fully appreciated. We show here that these antibiotics induce a mitonuclear protein imbalance through their effects on mitochondrial translation, an effect that likely reflects the evolutionary relationship between mitochondria and proteobacteria. Even at low concentrations, tetracyclines induce mitochondrial proteotoxic stress, leading to changes in nuclear gene expression and altered mitochondrial dynamics and function in commonly used cell types, as well as worms, flies, mice, and plants. Given that tetracyclines are so widely applied in research, scientists should be aware of their potentially confounding effects on experimental results. Furthermore, these results caution against extensive use of tetracyclines in livestock due to potential downstream impacts on the environment and human health
AB - In recent years, tetracyclines, such as doxycycline, have become broadly used to control gene expression by virtue of the Tet-on/Tet-off systems. However, the wide range of direct effects of tetracycline use has not been fully appreciated. We show here that these antibiotics induce a mitonuclear protein imbalance through their effects on mitochondrial translation, an effect that likely reflects the evolutionary relationship between mitochondria and proteobacteria. Even at low concentrations, tetracyclines induce mitochondrial proteotoxic stress, leading to changes in nuclear gene expression and altered mitochondrial dynamics and function in commonly used cell types, as well as worms, flies, mice, and plants. Given that tetracyclines are so widely applied in research, scientists should be aware of their potentially confounding effects on experimental results. Furthermore, these results caution against extensive use of tetracyclines in livestock due to potential downstream impacts on the environment and human health
U2 - https://doi.org/10.1016/j.celrep.2015.02.034
DO - https://doi.org/10.1016/j.celrep.2015.02.034
M3 - Article
C2 - 25772356
SN - 2211-1247
VL - 10
SP - 1681
EP - 1691
JO - Cell reports
JF - Cell reports
IS - 10
ER -