The clinically active BTK inhibitor PCI-32765 targets B-cell receptor- and chemokine-controlled adhesion and migration in chronic lymphocytic leukemia

Martin F. M. de Rooij, Annemieke Kuil, Christian R. Geest, Eric Eldering, Betty Y. Chang, Joseph J. Buggy, Steven T. Pals, Marcel Spaargaren

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480 Citations (Scopus)

Abstract

Small-molecule drugs that target the B-cell antigen receptor (BCR) signalosome show clinical efficacy in the treatment of B-cell non-Hodgkin lymphoma. These agents, including the Bruton tyrosine kinase (BTK) inhibitor PCI-32765, display an unexpected response in patients with chronic lymphocytic leukemia (CLL): a rapid and sustained reduction of lymphadenopathy accompanied by transient lymphocytosis, which is reversible upon temporary drug deprivation. We hypothesized that this clinical response reflects impaired integrin-mediated adhesion and/or migration. Here, we show that PCI-32765 strongly inhibits BCR-controlled signaling and integrin alpha(4)beta(1)-mediated adhesion to fibronectin and VCAM-1 of lymphoma cell lines and primary CLL cells. Furthermore, PCI-32765 also inhibits CXCL12-, CXCL13-, and CCL19-induced signaling, adhesion, and migration of primary CLLcells. Our data indicate that inhibition of BTK by PCI-32765 overcomes BCR- and chemokine-controlled integrin-mediated retention and homing of malignant B cells in their growth-and survival-supporting lymph node and bone marrow microenvironment, which results in clinically evident CLL regression. (Blood. 2012;119(11):2590-2594)
Original languageEnglish
Pages (from-to)2590-2594
JournalBlood
Volume119
Issue number11
DOIs
Publication statusPublished - 2012

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