The HLA-A*0201-restricted H-Y antigen contains a posttranslationally modified cysteine that significantly affects T cell recognition

L Meadows, W Wang, J M den Haan, E Blokland, C Reinhardus, J W Drijfhout, J Shabanowitz, R Pierce, A I Agulnik, C E Bishop, D F Hunt, E Goulmy, V H Engelhard

Research output: Contribution to journalArticleAcademicpeer-review

254 Citations (Scopus)

Abstract

A peptide recognized by two cytotoxic T cell clones specific for the human minor histocompatibility antigen H-Y and restricted by HLA-A*0201 was identified. This peptide originates from SMCY, as do two other H-Y epitopes, supporting the importance of this protein as a major source of H-Y determinants in mice and humans. In naturally processed peptides, T cells only recognize posttranslationally altered forms of this peptide that have undergone modification of a cysteine residue in the seventh position. One of these modifications involves attachment of a second cysteine residue via a disulfide bond. This modification has profound effects on T cell recognition and also occurs in other class I MHC-associated peptides, supporting its general importance as an immunological determinant.

Original languageEnglish
Pages (from-to)273-81
Number of pages9
JournalImmunity
Volume6
Issue number3
Publication statusPublished - Mar 1997

Keywords

  • Animals
  • Artifacts
  • Cells, Cultured
  • Clone Cells
  • Cysteine/genetics
  • Epitopes/immunology
  • Female
  • H-Y Antigen/genetics
  • HLA-A2 Antigen/genetics
  • Humans
  • Male
  • Mice
  • Peptides/immunology
  • Protein Binding/immunology
  • Protein Processing, Post-Translational/immunology
  • Receptors, Antigen, T-Cell/immunology
  • T-Lymphocytes, Cytotoxic/immunology

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