The non-toxic and biodegradable adjuvant Montanide ISA 720/CpG can replace Freund's in a cancer vaccine targeting ED-B--a prerequisite for clinical development

Elisabeth J M Huijbers, Julia Femel, Karl Andersson, Hanna Björkelund, Lars Hellman, Anna-Karin Olsson

Research output: Contribution to journalArticleAcademicpeer-review

21 Citations (Scopus)

Abstract

We have recently shown that immunization against the extra domain-B (ED-B) of fibronectin, using Freund's adjuvant, reduces tumor growth in mice by 70%. In the present study we compare the immune response generated against ED-B using the non-toxic and biodegradable adjuvant Montanide ISA 720/CpG with the response elicited by Freund's adjuvant. Montanide ISA 720/CpG induced anti-ED-B antibodies with higher avidity and less variable levels between individuals than Freund's. Moreover, the duration of the immune response was longer and the generation of anti-ED-B antibodies in naïve mice was faster, when Montanide ISA 720/CpG was used. We conclude that it is possible to replace the mineral oil based adjuvant Freund's with an adjuvant acceptable for human use, which is a prerequisite for transfer of the ED-B vaccine to the clinic.

Original languageEnglish
Pages (from-to)225-30
Number of pages6
JournalVaccine
Volume30
Issue number2
DOIs
Publication statusPublished - 5 Jan 2012

Keywords

  • Adjuvants, Immunologic/administration & dosage
  • Animals
  • Antibodies, Neoplasm/blood
  • Cancer Vaccines/administration & dosage
  • Female
  • Fibronectins/antagonists & inhibitors
  • Freund's Adjuvant/administration & dosage
  • Mannitol/administration & dosage
  • Mice
  • Mice, Inbred C57BL
  • Oleic Acids/administration & dosage
  • Protein Structure, Tertiary

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