@article{d6d9f33f8b3f44cea84e41afb419e1bd,
title = "The profiling of microbiota in vaginal swab samples using 16S rRNA gene sequencing and IS-pro analysis",
abstract = "BACKGROUND: 16S rRNA gene sequencing is currently the most common way of determining the composition of microbiota. This technique has enabled many new discoveries to be made regarding the relevance of microbiota to the health and disease of the host. However, compared to other diagnostic techniques, 16S rRNA gene sequencing is fairly costly and labor intensive, leaving room for other techniques to improve on these aspects. RESULTS: The current study aimed to compare the output of 16S rRNA gene sequencing to the output of the quick IS-pro analysis, using vaginal swab samples from 297 women of reproductive age. 16S rRNA gene sequencing and IS-pro analyses yielded very similar vaginal microbiome profiles, with a median Pearson's R2 of 0.97, indicating a high level of similarity between both techniques. CONCLUSIONS: We conclude that the results of 16S rRNA gene sequencing and IS-pro are highly comparable and that both can be used to accurately determine the vaginal microbiota composition, with the IS-pro analysis having the benefit of rapidity.",
keywords = "16S rRNA gene sequencing, IS-pro, Vaginal microbiota",
author = "M. Singer and R. Koedooder and Bos, {M. P.} and L. Poort and S. Schoenmakers and Savelkoul, {P. H. M.} and Laven, {J. S. E.} and {de Jonge}, {J. D.} and Morr{\'e}, {S. A.} and Budding, {A. E.}",
note = "Funding Information: The authors would like to acknowledge the efforts of dr. R van Houdt and C. van der Veer from the department of Medical Microbiology at Amsterdam UMC, location VUmc, Amsterdam in the setup of the 16S rRNA gene sequencing protocol. Additionally, the authors greatly appreciate the collaborating centers: Radboud University Medical Center, Erasmus University Medical Center, Isala kliniek Zwolle, VU University Medical Center, Sint Elisabeth Ziekenhuis, VivaNeo Medisch Centrum Kinderwens, University Medical Center Utrecht, and University Medical Center Maastricht for the timely sample inclusions. Funding Information: This study was financed by NGI Pre-Seed 2014–2016, RedMedTech Discovery Fund 2014–2017, STW Valorisation grant 1 2014–2015, STW Take-off early phase trajectory 2015–2016, Eurostars VALBIOME grant (reference number: 8884), MIND subsidy. Publisher Copyright: {\textcopyright} 2021, The Author(s). Copyright: Copyright 2021 Elsevier B.V., All rights reserved.",
year = "2021",
month = dec,
doi = "https://doi.org/10.1186/s12866-021-02149-7",
language = "English",
volume = "21",
pages = "100",
journal = "BMC microbiology",
issn = "1471-2180",
publisher = "BioMed Central",
number = "1",
}