The Saccharomyces cerevisiae ABC subfamily D transporter Pxa1/Pxa2p co-imports CoASH into the peroxisome

Research output: Contribution to journalArticleAcademicpeer-review

15 Citations (Scopus)

Abstract

ATP-binding cassette (ABC) subfamily D transporters are important for the uptake of fatty acids and other beta-oxidation substrates into peroxisomes. Genetic and biochemical evidence indicates that the transporters accept fatty acyl-coenzyme A that is cleaved during the transport cycle and then re-esterified in the peroxisomal lumen. However, it is not known whether free coenzyme A (CoA) is released inside or outside the peroxisome. Here we have used Saccharomyces cerevisiae and isolated peroxisomes to demonstrate that free CoA is released in the peroxisomal lumen. Thus, ABC subfamily D transporter provide an import pathway for free CoA that controls peroxisomal CoA homeostasis and tunes metabolism according to the cell’s demands.
Original languageEnglish
Pages (from-to)763-772
Number of pages10
JournalFEBS letters
Volume595
Issue number6
Early online date2020
DOIs
Publication statusPublished - Mar 2021

Keywords

  • ABC transporter
  • acyl-CoA
  • coenzyme A
  • coenzyme homeostasis
  • peroxisome
  • thioesterase

Cite this