TY - JOUR
T1 - The use of chemogenetics in behavioural neuroscience
T2 - receptor variants, targeting approaches and caveats
AU - Campbell, Erin J.
AU - Marchant, Nathan J.
PY - 2018/4/1
Y1 - 2018/4/1
N2 - The last decade has seen major advances in neuroscience tools allowing us to selectively modulate cellular pathways in freely moving animals. Chemogenetic approaches such as designer receptors exclusively activated by designer drugs (DREADDs) permit the remote control of neuronal function by systemic drug administration. These approaches have dramatically advanced our understanding of the neural control of behaviour. Here, we review the different techniques and genetic approaches available for the restriction of chemogenetic receptors to defined neuronal populations. We highlight the use of a dual virus approach to target specific circuitries and the effectiveness of different routes of administration of designer drugs. Finally, we discuss the potential caveats associated with DREADDs including off-target effects of designer drugs, the effects of chronic chemogenetic receptor activation and the issue of collateral projections associated with DREADD activation and inhibition.
AB - The last decade has seen major advances in neuroscience tools allowing us to selectively modulate cellular pathways in freely moving animals. Chemogenetic approaches such as designer receptors exclusively activated by designer drugs (DREADDs) permit the remote control of neuronal function by systemic drug administration. These approaches have dramatically advanced our understanding of the neural control of behaviour. Here, we review the different techniques and genetic approaches available for the restriction of chemogenetic receptors to defined neuronal populations. We highlight the use of a dual virus approach to target specific circuitries and the effectiveness of different routes of administration of designer drugs. Finally, we discuss the potential caveats associated with DREADDs including off-target effects of designer drugs, the effects of chronic chemogenetic receptor activation and the issue of collateral projections associated with DREADD activation and inhibition.
UR - http://www.scopus.com/inward/record.url?scp=85042411371&partnerID=8YFLogxK
U2 - https://doi.org/10.1111/bph.14146
DO - https://doi.org/10.1111/bph.14146
M3 - Review article
C2 - 29338070
SN - 0007-1188
VL - 175
SP - 994
EP - 1003
JO - British journal of pharmacology
JF - British journal of pharmacology
IS - 7
ER -